REGULATION OF DEVELOPMENT OF PEPTIDE NEURONS IN GUT

这个项目的目的是描述5-羟色胺能神经元的发育。 在雏鸡肠道中的神经元，并表征肠道运动的发育 通过测定正常雏鸡和处理过的雏鸡的蠕动反射 5-羟色胺能神经元特有的神经毒素(5，7-二羟色胺)。 5-羟色胺能神经元发育的放射自显影研究 使用生化技术学习出现的时间和位置的技术 学习外观的合成和释放，并用机械换能器 学习感受器的外观。蠕动反射的发展 将用机械换能器在分离的肠道节段中进行研究 和接受神经毒素治疗的小鸡。最后，作为行动基础的机制 5-羟色胺能神经元将在细胞水平上结合 方法包括活体染色、细胞内记录、细胞内标记 辣根过氧化物酶，以及光镜和电子显微镜。 所获得的信息可能有助于理解潜在的机制 肠道的病理生理状态，如麻痹性肠梗阻和巨结肠。

The aim of this project is to characterize the development of the serotonergic neuron in the chick gut and to characterize the development of gut motility as measured by the peristaltic reflex in normal chicks and in chicks treated with neurotoxins specific for serotonergic neurons (5, 7 dihydroxytryptamine). Development of serotonergic neurons will be studied with autoradiographic techniques to learn time of appearance and location, with biochemical techniques to learn appearance of synthesis and release, and with mechanical transducers to learn the appearance of receptors. The development of the peristaltic reflex will be studied with mechanical transducers in isolated gut segments from normal and neurotoxin-treated chicks. Finally, the mechanisms underlying the action of serotonergic neurons will be studied on the cellular level by a combination of methods including vital staining, intracellular recording, intracellular marking with horseradish peroxidase, and light and electron microscopy. Information obtained may be useful in understanding the mechanisms underlying pathophysiological conditions of the gut such as paralytic ileus and megacolon.