LYSOGENY AND BACTERIOPHAGE P1

温和相P1是不寻常的，因为它的原噬菌体是质粒。 的 提出的研究是遗传和生物化学的方法，以了解更多关于 决定病毒基因何时表达的调控机制， 采用哪种DNA复制模式（质粒或病毒）。 具体地说， 涉及重复感染免疫和原噬菌体抑制的表达的研究 都计划好了 由于其染色体外的形式，原噬菌体P1作为一种 抗生素抗性质粒和动物病毒的模型， 致癌性EB病毒（EBV）。 EBV的质粒DNA形式存在于 Burkitt淋巴瘤细胞和Raji细胞系中，因此似乎是 参与了转型。 我们希望， P1中控制前噬菌体维持的机制将有助于 了解EBV和其他类似病毒的质粒形式的维持 动物病毒 此外，P1中的位点特异性重组系统似乎涉及 在基因表达调控中的作用。 由于癌病毒整合到细胞内， DNA在转化过程中通过在表面上的特定位点重组而形成的 封闭的共价环状病毒DNA拷贝，这是预期比理解 噬菌体P1中这种现象的研究可能有助于理解 在这些病毒中位点特异性重组的重要性。

The temperate phase P1 is unusual because its prophage is a plasmid. The studies proposed are genetic and biochemical approaches to learning more about the regulatory mechanisms that determine when viral genes are expressed and which mode of DNA replication (plasmid or viral) is employed. Specifically, studies involving expression of superinfection immunity and prophage repression are planned. Because of its extrachromosomal form, prophage P1 serves as a model both for antibiotic resistance plasmids and for animal viruses like the carcinogenic Epstein-Barr virus (EBV). The plasmid DNA form of EBV is found in Burkitt lymphoma cells and in the Raji cell line, and thus appears to be involved in transformation. We hope that a better understanding of the mechanisms in P1 that control maintenance of the prophages will help in understanding the maintenance of the plasmid form of EBV and other similar animal viruses. In addition, a site-specific recombination system in P1 appears to be involved in regulation of gene expression. Since oncornaviruses integrate into cellular DNA during transformation by recombination at a specific site on an apparently closed covalent circular viral DNA copy, it is anticipated than an understanding of this phenomenon in bacteriophage P1 may be useful for understanding the importance of site-specific recombination in such viruses.