OPTICAL STUDIES OF T-SYSTEM FUNCTION IN E-C COUPLING

骨骼肌激活(兴奋-收缩耦合)涉及信号 通过特殊的膜连接(三联体)传播 横管(T系统)和肌浆网。目的 这项研究的特点是描述了一种非线性势能变化 可能发生在T系统的膜上，可能是在三元连接处。这 这一现象是私家侦探最近使用电位法染料和 反映了发生的瞬时静电电位变化 去极化时，与兴奋收缩密切相关 耦合。这项研究将考察这一现象与 Q伽马运动，现在被认为反映了 T系统中介导连接的电压敏感分子 变速箱。此外，它还将检查细胞的生理功能 这一现象及其与致病分子机制的关系 结合式耦合。 这些问题将通过详细的比较来解决 静电势随电荷运动呈非线性变化。这个 测量将使用单个电压钳制的肌肉纤维进行 用电位法染料染色并安装在实验装置中 它允许同时测量T系统光信号和电荷 有动静。 这项研究有助于更好地理解正常的发病机制。 肌肉激活。此外，它与肌肉营养不良症也有广泛的相关性。 这涉及到兴奋-收缩耦合的异常，以及 肌松药丹曲林钠离子等治疗剂的设计 具体来说，改变这一过程。

Skeletal muscle activation (excitation-contraction coupling) involves signal transmission across specialized membrane junctions (triads) that join the transverse tubules (T-system) with the Sarcoplasmic Reticulum. The purpose of this research is to characterize a non-linear potential change that occurs in the T-system membranes, presumably at the triadic junctions. This phenomenon was recently discovered by the P.I. using potentiometric dyes and was shown to reflect a transient electrostatic potential change that occurs upon depolarization and is closely associated with excitation-contraction coupling. The research will examine the relationship of this phenomenon to Q gamma movement, which is now thought to reflect a conformational change on the voltage-sensing molecule in the T-system that mediates junctional transmission. Additionally, it will examine the physiological function of this phenomenon and its relationship to the molecular mechanism of junctional coupling. These questions will be addressed by making detailed comparisons of the non-linear electrostatic potential change, with charge movement. The measurements will be performed using single voltage-clamped muscle fibers stained with potentiometric dyes and mounted in an experimental apparatus that allows simultaneous measurement of T-system optical signals and charge movement. This research can lead to a better understanding of the mechanisms of normal muscle activation. Also, it is broadly relevant to muscular dystrophies that involve an abnormality of excitation-contraction coupling and to the design of therapeutic agents such as the muscle relaxant Dantrolene Na+ that specifically alter this process.