MUSCLE SATELLITE CELL: REACTION TO DISEASE AND TRAUMA

有人建议深入研究由基因决定的肌病， 特别强调以下目标； 1.澄清(S)负责确定 遗传性营养不良(Dy)小鼠肌肉表达或不表达 营养不良表型。 2.对退化的抑制是否- 营养不良(Dy)肌肉出现的再生周期 暂时性失神经是这个营养不良突变体所独有的，或者 类似的修改将发生在其他不同的基因决定的 肌病(例如，MDX小鼠和营养不良的仓鼠)。 3.抑制退化的效果的测定- 营养不良(Dy)肌纤维的再生周期在发育中的作用 这些肌肉的肌卫星细胞的增殖性衰老。 Dy肌卫星增殖性衰老的检测 体外培养的细胞是有丝分裂能力耗尽的结果 这些细胞在疾病过程的早期阶段，或者 Dy肌的肌卫星细胞存在原发缺陷。 4.评估MDX中发生的再生程度 突变小鼠。肌卫星有丝分裂能力的评价 对MDX肌肉细胞进行克隆分析。

It is proposed to study, in depth, genetically determined myopathies, with particular emphasis on the following objectives; 1. Elucidation of the mechanism(s) responsible for determining whether genetically dystrophic (dy) murine muscle expresses or fails to express the dystrophic phenotype. 2. Determination of whether the inhibition of the degeneration- regeneration cycle which occurs when dystrophic (dy) muscle is transiently denervated is unique to this dystrophic mutant or whether a similar modification will occur in other diverse genetically determined myopathies (e.g., the mdx mouse and the dystrophic hamster). 3. Determination of the effect of inhibition of the degeneration- regeneration cycle of dystrophic (dy) myofibers on the development of proliferative senescence of myosatellite cells of these muscles. Determination whether the proliferative senescence of dy myosatellite cells in vitro is a result of the exhaustion of the mitotic capability of these cells during the early stage of the disease process or whether there is a primary defect in the myosatellite cells of dy muscles. 4. Evaluation of the extent of regeneration which occurs in the mdx mutant mouse. Evaluation of the mitotic capability of the myosatellite cells of the mdx muscle using clonal analysis.