MYOGENESIS SUBSEQUENT TO DENERVATION

去神经支配后的肌生成

基本信息

项目摘要

In vivo myogenesis occurs simultaneously with the development and maturation of the nervous system. Recently described techniques permit experimental manipulation of mouse fetuses without interfering with fetal viability and maturation. These techniques will be applied to laser ablate the spinal cords of young fetuses and to cut the sciatic nerves of older fetuses, permitting the evaluation of the effects of denervation (at various developmental periods) on the development and maturation of mammalian myofibers. Evaluation will be made with electron microscopy and morphometry. We also will evaluate the effect of fetal denervation on the expression of muscle specific genes encoding the various myosin and actin isoforms in mouse hindlimb muscles with in situ hybridization and with immunohistochemistry, using an immunohistochemical technique which will permit differentiation of isoforms present in primary versus secondary myotubes. The time course of expression of genes encoding the fetal and adult forms of creatine kinase and the genes coding for the various subunits of the acetylcholine receptor (both the fetal and adult receptors) in innervated hindlimb muscles will be determined with in situ hybridization. We then will evaluate the effects of denervation on the expression of these genes and the genes encoding the myogenic factors MyoD1 and myogenin. The proposed studies will allow us to assess the effect of environment on the innate genetic program of developing muscle: 1) It will permit assessment of cell interactions and of how the neuron may play a role in commitment, differentiation, or change the behavior of muscle cells. 2) I will permit determination of the extent to which nerve regulation is required for the maintenance of muscle phenotypes in the course of development. Information generated from the proposed studies will increase our "...understanding of the cellular and molecular processes involved in muscle development ..." and "... will be invaluable in the design and implementation of techniques to repair muscles that are damaged or nonfunctional due to genetic or environmental causes".
在体内,肌肉发生与肌肉的发育同时发生

项目成果

期刊论文数量(10)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Ablation of the fetal mouse spinal cord: the effect on soleus muscle cytoarchitecture.
胎儿小鼠脊髓的消融:对比目鱼肌细胞结构的影响。
Opioid gene expression in the developing and adult rat heart.
阿片类药物基因在发育中和成年大鼠心脏中的表达。
Reinnervation of murine muscle following fetal sciatic nerve transection.
胎儿坐骨神经横断后小鼠肌肉的神经再支配。
  • DOI:
    10.1002/neu.480220902
  • 发表时间:
    1991
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Mallonga,RL;Ontell,M
  • 通讯作者:
    Ontell,M
Effect of chronic denervation and denervation-reinnervation on cytoplasmic creatine kinase transcript accumulation.
慢性去神经支配和去神经支配-再神经支配对细胞质肌酸激酶转录物积累的影响。
  • DOI:
    10.1002/neu.1027
  • 发表时间:
    2001
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Washabaugh,CH;Ontell,MP;Kant,JA;Daood,MJ;Watchko,JF;Watkins,SC;Ontell,M
  • 通讯作者:
    Ontell,M
Contractile protein gene expression in primary myotubes of embryonic mouse hindlimb muscles.
胚胎小鼠后肢肌肉初级肌管中收缩蛋白基因的表达。
  • DOI:
    10.1242/dev.117.4.1435
  • 发表时间:
    1993
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Ontell,M;Ontell,MP;Sopper,MM;Mallonga,R;Lyons,G;Buckingham,M
  • 通讯作者:
    Buckingham,M
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Marcia R. Ontell其他文献

Marcia R. Ontell的其他文献

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{{ truncateString('Marcia R. Ontell', 18)}}的其他基金

MYOGENIC FACTORS: MUSCLE MATURATION AND REGENERATION
生肌因素:肌肉成熟和再生
  • 批准号:
    6652674
  • 财政年份:
    2000
  • 资助金额:
    $ 23.38万
  • 项目类别:
MYOGENIC FACTORS: MUSCLE MATURATION AND REGENERATION
生肌因素:肌肉成熟和再生
  • 批准号:
    6190628
  • 财政年份:
    2000
  • 资助金额:
    $ 23.38万
  • 项目类别:
MYOGENIC FACTORS: MUSCLE MATURATION AND REGENERATION
生肌因素:肌肉成熟和再生
  • 批准号:
    6375287
  • 财政年份:
    2000
  • 资助金额:
    $ 23.38万
  • 项目类别:
MYOGENIC FACTORS: MUSCLE MATURATION AND REGENERATION
生肌因素:肌肉成熟和再生
  • 批准号:
    6534490
  • 财政年份:
    2000
  • 资助金额:
    $ 23.38万
  • 项目类别:
MYOGENIC FACTORS: MUSCLE MATURATION AND REGENERATION
生肌因素:肌肉成熟和再生
  • 批准号:
    6797228
  • 财政年份:
    2000
  • 资助金额:
    $ 23.38万
  • 项目类别:
MYOGENESIS SUBSEQUENT TO DENERVATION
去神经支配后的肌生成
  • 批准号:
    2392396
  • 财政年份:
    1990
  • 资助金额:
    $ 23.38万
  • 项目类别:
MAMMALIAN MYOGENESIS SUBSEQUENT TO FETAL DENERVATION
胎儿去神经后的哺乳动物肌生成
  • 批准号:
    3326824
  • 财政年份:
    1990
  • 资助金额:
    $ 23.38万
  • 项目类别:
MYOGENESIS SUBSEQUENT TO DENERVATION
去神经支配后的肌生成
  • 批准号:
    2199668
  • 财政年份:
    1990
  • 资助金额:
    $ 23.38万
  • 项目类别:
MAMMALIAN MYOGENESIS SUBSEQUENT TO FETAL DENERVATION
胎儿去神经后的哺乳动物肌生成
  • 批准号:
    3326827
  • 财政年份:
    1990
  • 资助金额:
    $ 23.38万
  • 项目类别:
MAMMALIAN MYOGENESIS SUBSEQUENT TO FETAL DENERVATION
胎儿去神经后的哺乳动物肌生成
  • 批准号:
    3326828
  • 财政年份:
    1990
  • 资助金额:
    $ 23.38万
  • 项目类别:

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