MOLECULAR BASIS OF CELLULAR ADHESIVENESS

细胞粘附性的分子基础

• 批准号: 3163974
• 负责人: FREDERICK GRINNELL
• 金额: $ 16.79万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-06-01 至 1987-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3163974
• 关键词: WI38 cell; affinity chromatography; blood proteins; cell adhesion; cell cell interaction; cell transformation; crosslink; electrofocusing; electron microscopy; embryo /fetus tissue /cell culture; fibroblasts; human tissue; immunofluorescence technique; infant animal; ligands; membrane activity; membrane reconstitution /synthesis; membrane structure; neoplasm /cancer genetics; neoplasm /cancer immunology; neoplastic transformation; peptidases; radiotracer; tissue /cell culture; tritium; ultraviolet spectrometry

The long-term goal of this research project is to understand the mechanism of cell adhesion, and involves a multidisciplinary approach using biochemical, immunological, and microscopic methods. During the past year we have characterized the FN-2 BHK cell adhesion variant and found that it is deficient in the cell-substratum contact process. That is, cell adhesion receptors are present on the surface of these cells, but the receptors cannot reorganize so as to engage in cooperative interactions. A high molecular weight glycoprotein found on parental cells is absent from the FN-2 variant and will be characterized further in the future. We also have prepared 5 monoclonal antibodies to BHK cells and are in the process of studying the effects of these antibodies on cell adhesion. Analysis of cell cytoskeletal responses to different sized fibronectin-coated beads revealed reorganization of actin and alpha actinin in response to the binding of large (5 micron beads) but not small (1 micron beads). This reorganization appeared to reflect differences in cell deformation in response to the beads. The large beads induced the formation of large cell ruffles while the small beads interacted with cell microvilli. Studies are now underway to determine whether different subsets of cell membrane coponents interact with the large and small beads. (A)

本研究项目的长期目标是了解 的细胞粘附，并涉及多学科的方法， 生物化学、免疫学和显微镜方法。 在过去一年中 我们已经鉴定了FN-2 BHK细胞粘附变体，并发现它 缺乏细胞-基质接触过程。 也就是说， 粘附受体存在于这些细胞的表面上，但是 受体不能重组以参与合作性相互作用。 一 在亲本细胞上发现的高分子量糖蛋白不存在于 FN-2的变体，并将在未来进一步表征。 我们也 已经制备了5种针对BHK细胞的单克隆抗体，并正在进行中 研究这些抗体对细胞粘附的影响。 分析 细胞骨架对不同大小纤连蛋白包被珠的反应 揭示了肌动蛋白和α-肌动蛋白的重组， 结合大的（5微米珠）但不结合小的（1微米珠）。 这 重组似乎反映了细胞变形的差异， 对珠子的反应。 大珠诱导大细胞的形成 褶皱，而小珠与细胞微绒毛相互作用。 研究是 现在正在进行的是确定不同的细胞膜亚群 组分与大珠和小珠相互作用。 （一）