MOLECULAR BASIS OF CELLULAR ADHESIVENESS

该研究项目的长期目标是了解其机制 细胞粘附，并涉及使用多学科方法 生物化学、免疫学和显微镜方法。 在过去的一年里 我们对 FN-2 BHK 细胞粘附变体进行了表征，发现它 细胞与基质的接触过程存在缺陷。 也就是说，细胞 粘附受体存在于这些细胞的表面，但是 受体不能重组以进行合作相互作用。 一个 亲本细胞上发现的高分子量糖蛋白不存在 FN-2 变体，并将在未来进一步表征。 我们也 已制备5种针对BHK细胞的单克隆抗体，正在进行中 研究这些抗体对细胞粘附的影响。 分析 细胞骨架对不同尺寸纤连蛋白包被珠的反应 揭示了肌动蛋白和α肌动蛋白的重组响应 结合大（5 微米珠）但不结合小（1 微米珠）。 这个 重组似乎反映了细胞变形的差异 对珠子的反应。 大珠诱导大细胞的形成 当小珠子与细胞微绒毛相互作用时，会产生褶皱。 研究是 现在正在进行以确定细胞膜的不同子集是否 成分与大珠和小珠相互作用。 （一个）

The long-term goal of this research project is to understand the mechanism of cell adhesion, and involves a multidisciplinary approach using biochemical, immunological, and microscopic methods. During the past year we have characterized the FN-2 BHK cell adhesion variant and found that it is deficient in the cell-substratum contact process. That is, cell adhesion receptors are present on the surface of these cells, but the receptors cannot reorganize so as to engage in cooperative interactions. A high molecular weight glycoprotein found on parental cells is absent from the FN-2 variant and will be characterized further in the future. We also have prepared 5 monoclonal antibodies to BHK cells and are in the process of studying the effects of these antibodies on cell adhesion. Analysis of cell cytoskeletal responses to different sized fibronectin-coated beads revealed reorganization of actin and alpha actinin in response to the binding of large (5 micron beads) but not small (1 micron beads). This reorganization appeared to reflect differences in cell deformation in response to the beads. The large beads induced the formation of large cell ruffles while the small beads interacted with cell microvilli. Studies are now underway to determine whether different subsets of cell membrane coponents interact with the large and small beads. (A)