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FILTERABLE AGENTS AND TUMOR INDUCTION IN MICE

可过滤剂和小鼠肿瘤诱导

基本信息

批准号：
3163347
负责人：
BEATRIZ G. POGO
金额：
$ 20.96万
依托单位：
MOUNT SINAI SCHOOL OF MEDICINE OF CUNY
依托单位国家：
美国
项目类别：
财政年份：
1974
资助国家：
美国
起止时间：
1974-09-01 至 1988-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3163347
关键词：
DNA methylation Friend virus Retroviridae disease erythroleukemia gene expression genetic mapping genetic markers molecular cloning neoplasm /cancer genetics neoplasm /cancer immunology nucleic acid sequence tissue /cell culture viral leukemogenesis virus classification virus genetics

项目摘要

The Friend leukemia viruses are a family of variant viruses that induce similar but not identical patterns of disease. The wild type causes an erythroleukemia in adult mice, either associated with anemia (FLV-A) as was the original isolate, or with polycythemia (FLV-P). The FLV-P strains consist of a complex of FLV-A, the defective spleen focus-forming virus (SFFV) and additional genetic components of endogenous mus sequences. These variant viruses may have emerged as a result of recombinant events. We plan a comparative study of the molecular properties and biological activities of the leukemogenic FLV-A and FLV-P virus strains produced in vivo in the leukemic mouse, and the attenuated virus produced in vitro by our chronically infected erythroleukemia cell lines. The integrated and free viral DNA of each strain will be characterized by restriction enzyme analysis. Using FLV and SFFV probes obtained from different laboratories and probes from clone virus of our strains, the integration patterns and the expression of the viral genome will be examined. The level of methylation of the pro-viral and viral genes will aso be investigated, since gene activity may be influenced by the degree of methylation. We will also seek to define the role of the virion protein kinase and endonuclease. In characterizing the RNA, protein and enzymatic activities of the purified virions, we hope to obtain a profile which will identify each strain. This would be extremely useful in comparing alterations in the properties of the infecting virus with that of the virus recovered from the leukemic animal, in view of the readiness with which recombination occurs with components of endogenous viruses of the cell. It may also be possible to associate these changes with changes in malignant potential. Information sought from this proposed work can be of potential significance to explain the molecular basis of leukemogenicity. A better understanding of the mechanisms involved in the development of leukemia is fundamental for the prevention and treatment of the disease.

Friend 白血病病毒是一个变异病毒家族，可诱导相似但不相同的疾病模式。野生型导致成年小鼠的红白血病，要么与贫血（FLV-A）相关原始分离株，或伴有红细胞增多症（FLV-P）。 FLV-P菌株由FLV-A复合体组成，FLV-A是有缺陷的脾病灶形成病毒 (SFFV) 和内源 mus 序列的其他遗传成分。这些变异病毒可能是重组事件的结果。我们计划对分子特性和生物特性进行比较研究产生的致白血病 FLV-A 和 FLV-P 病毒株的活性白血病小鼠体内，以及体外产生的减毒病毒我们的慢性感染红白血病细胞系。每个毒株的整合和游离病毒 DNA 的特征为限制性内切酶分析。使用从以下位置获得的 FLV 和 SFFV 探针不同的实验室和探针来自我们菌株的克隆病毒，病毒基因组的整合模式和表达将是检查了。前病毒和病毒基因的甲基化水平将也需要进行研究，因为基因活性可能会受到甲基化。我们还将寻求定义病毒体蛋白的作用激酶和核酸内切酶。表征 RNA、蛋白质和酶纯化的病毒体的活性，我们希望获得一个概况，这将识别每种菌株。这对于比较非常有用感染病毒的特性与病毒本身的特性发生改变从白血病动物身上康复，考虑到已做好准备重组与细胞内源病毒的成分发生。它也可能将这些变化与恶性变化联系起来。潜在的。从这项拟议工作中寻求的信息可能具有潜在意义解释白血病发生的分子基础。更好的理解参与白血病发展的机制是根本性的用于疾病的预防和治疗。

项目成果

期刊论文数量（19）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Induction of differentiation in Friend erythroleukemia cells with dimethyl sulfoxide, hexamethylene bisacetamide and sodium butyrate is not accompanied by changes in proviral DNA or its expression.

用二甲亚砜、六亚甲基双乙酰胺和丁酸钠诱导 Friend 红白血病细胞分化并不伴随原病毒 DNA 或其表达的变化。

DOI：
10.1016/0168-1702(86)90056-0
发表时间：
1986
期刊：
Virus research
影响因子：
5
作者：
Zajac-Kaye,M;Brown,E;Friend,C
通讯作者：
Friend,C

Hemin-independent control of globin synthesis in Friend erythroleukemia cells induced to differentiate.

诱导分化的 Friend 红白血病细胞中珠蛋白合成的血红素独立控制。

DOI：
10.1073/pnas.79.6.1839
发表时间：
1982
期刊：
Proceedings of the National Academy of Sciences of the United States of America
影响因子：
11.1
作者：
Stringer,EA;Friend,C
通讯作者：
Friend,C

Altered RNA/protein ratio associated with the induction of differentiation of Friend erythroleukemia cells.

RNA/蛋白质比例的改变与 Friend 红白血病细胞分化的诱导相关。

DOI：
10.1073/pnas.78.6.3882
发表时间：
1981
期刊：
Proceedings of the National Academy of Sciences of the United States of America
影响因子：
11.1
作者：
Harel,L;Blat,C;Lacour,F;Friend,C
通讯作者：
Friend,C

The molecular pathology of Friend erythroleukemia virus strains. An overview.

弗兰德红白血病病毒株的分子病理学。