ROLE OF 1,25 DIHYDROXYVITAMIN D DURING FETAL LIFE
ROLE OF 1,25 DIHYDROXYVITAMIN D DURING FETAL LIFE
批准号:
3157620
负责人:
RICHARDUS ROSS
金额:
$11.39万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1986
资助国家:
美国
项目状态:
已结题
起止时间:
1986-01-01 至 1988-12-31
关键词:
1,25 dihydroxycholecalciferol atomic absorption spectrometry bone metabolism disorder embryo /fetus high performance liquid chromatography hypocalcemia kidney function kidney metabolism longitudinal animal study nephrectomy parathyroid hormones pregnancy circulation premature infant animal radioimmunoassay respiratory gas analyzer scintillation spectrometry vitamin biosynthesis vitamin metabolism vitamin therapy
中文摘要
许多早产儿在出生后的头24小时内出现低钙血症
生活。在这些人中,20%可能有后来的骨骼
脱矿(骨量减少、软骨病、长骨骨折)。这个
潜在的机制尚不清楚,但可能涉及异常的维生素D
新陈代谢。本提案的目的是帮助澄清
维生素D的激素形式,即1,25二羟基维生素D
(1,25(OH)2D)在胎儿宫内生活中的矿物质经济。
将解决以下具体问题:1)什么是
胎儿肾脏和胎盘对胎儿的相对贡献
宫内循环中的1,25(OH)2D浓度?2)
正常胎儿循环中正常所需的1,25(OH)2D浓度
宫内矿物质代谢?3)胎盘和胎骨
胎儿时期1,25(OH)2D作用的反应靶器官?
在我们研究的第一阶段,独生羊胚胎将在
妊娠110天(第145期)测定1,25(OH)2D合成
胎儿-胎盘单位的容量。这将通过测量以下各项来完成
1,25(OH)2D和1,25(OH)2D对胎儿的产生量和代谢清除率的影响
体内胎盘合成率的测定
胎盘灌注法。正常妊娠相关的变化
还将评估血清矿物质和钙调节激素。
胎盘正常时,纵向妊娠110天至138天
将测定矿物质转移率和胎儿矿物质利用率。
在我们的第二阶段研究中,我们将使用双侧肾切除的胎儿来
测定胎儿肾脏1,25(OH)2D合成能力丧失的影响
胎儿血清1,25(OH)2D和矿物质浓度、胎盘矿物质
转移率和胎儿矿物质利用率。基于我们之前的
研究表明,胎儿肾切除会导致胎儿低钙血症,
高磷血症和胎儿血清1,25(OH)2D浓度降低。我们
假设这些变化是由1,25(OH)2D介导的减少引起的
胎盘矿物质转移和破坏正常的骨矿化和
将通过外源性1,25(OH)2D治疗在很大程度上得到纠正。
这些联合研究将提供有价值的洞察功能
1,25(OH)2D在胎儿生命中的作用
早产儿低钙血症和骨脱矿。
英文摘要
Many premature infants develop hypocalcemia during the first 24 hours of
life. Of these, twenty percent may have subsequent skeletal
demineralization (osteopenia, rickets, long bone fractures). The
underlying mechanisms are unknown but may involve abnormal vitamin D
metabolism. The present proposal is designed to help clarify the role of
the hormonal form of vitamin D, namely, 1,25 dihydroxyvitamin D
(1,25(OH)2D) in the mineral economy of the fetus during intrauterine life.
The following specific questions will be addressed: 1) What are the
relative contributions of the fetal kidney and the placenta to fetal
circulating 1,25(OH)2D concentrations during intrauterine life? 2) Are
normal fetal circulating 1,25(OH)2D concentrations necessary for normal
intrauterine mineral metabolism? 3) Are the placenta and fetal bone
responsive target organs for the action of 1,25(OH)2D during fetal life?
In phase I of our study, singleton sheep fetuses will be instrumented at
110 days of gestation (term 145) to determine the 1,25(OH)2D synthetic
capacity of the fetal-placental unit. This will be done by measurement of
the fetal Production and Metabolic Clearance Rates of 1,25(OH)2D and by
measurement of the in vivo placental synthesis rate using an in vivo
placental perfusion technique. Normal gestationally related changes in
serum minerals and calcium regulating hormones will also be assessed
longitudinally from 110 days to 138 days of gestation when normal placental
mineral transfer and fetal mineral utilization rates will be determined.
In phase II of our study, we will use bilaterally nephrectomized fetuses to
determine the impact of loss of fetal renal 1,25(OH)2D synthetic capacity
on fetal serum 1,25(OH)2D and mineral concentrations, placental mineral
transfer and fetal mineral utilization rates. Based on our previous
studies, fetal nephrectomy will result in fetal hypocalcemia,
hyperphosphatemia and reduced fetal serum 1,25(OH)2D concentrations. We
hypothesize that these changes result from reduced 1,25(OH)2D-mediated
placental mineral transfer and disruption of normal bone mineralization and
will be largely corrected by treatment with exogenous 1,25(OH)2D therapy.
These combined studies will provide valuable insight into the functional
role of 1,25(OH)2D during fetal life, as it relates to the etiology of
hypocalcemia and bone demineralization in prematurity.
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ISOFLAVONE-RICH PASTA--CLINICAL AND COMMERCIAL POTENTIAL
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资助金额:$9.81万
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THE ROLE OF 1,25 DIHYDOXYVITAMIN D DURING FETAL LIFE
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批准号:3157622
-
项目类别:
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资助金额:$10.85万
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THE ROLE OF 1,25 DIHYDROXYVITAMIN D DURING FETAL LIFE
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批准号:3157621
-
项目类别:
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资助金额:$11.04万
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财政年份:1986
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负责人:RICHARDUS ROSS
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依托单位:
MATERNAL-FETAL MINERAL METABOLISM IN DIABETIC PREGNANCY
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批准号:3842627
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:RICHARDUS ROSS
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MATERNAL-FETAL MINERAL METABOLISM IN DIABETIC PREGNANCY
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资助金额:$0.0万
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财政年份:--
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INDUCTION AND MAINTENANCE OF DIABETES IN THE PREGNANT SHEEP
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批准号:3778861
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项目类别:
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资助金额:$0.0万
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财政年份:--
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依托单位:
INDUCTION AND MAINTENANCE OF DIABETES IN THE PREGNANT SHEEP
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:RICHARDUS ROSS
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MATERNAL-FETAL MINERAL METABOLISM IN DIABETIC PREGNANCY
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资助金额:$0.0万
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财政年份:--
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依托单位:
INDUCTION AND MAINTENANCE OF DIABETES IN THE PREGNANT SHEEP
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批准号:3756965
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:RICHARDUS ROSS
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MATERNAL-FETAL MINERAL METABOLISM IN DIABETIC PREGNANCY
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项目类别:
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资助金额:$0.0万
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财政年份:--
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依托单位:
MATERNAL-FETAL MINERAL METABOLISM IN DIABETIC PREGNANCY
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批准号:3857398
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资助金额:$0.0万
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财政年份:--
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负责人:RICHARDUS ROSS
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INDUCTION AND MAINTENANCE OF DIABETES IN THE PREGNANT SHEEP
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资助金额:$0.0万
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财政年份:--
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负责人:RICHARDUS ROSS
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资助金额:$0.0万
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财政年份:--
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负责人:RICHARDUS ROSS
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依托单位:
海外基金