MECHANISMS OF CONTROL OF MAMMALIAN CELL MULTIPLICATION

我们正在继续寻找定向的单抗 抗核抗原，其水平在G1期间发生显著变化 鸡胚成纤维细胞原代培养周期。我们已经改进了 酶联免疫吸附试验的敏感性。尽管如此，对几个 数千株杂交瘤上清液尚未产生阳性结果 结果。作为这个项目的一个分支，我们已经开始使用单克隆 寻找不同人群核蛋白水平差异的抗体 构成鸡胚胎的各种特化细胞 肾脏。已经发现了两种非常有趣的单抗， 目前正在努力识别这些抗原，并发现它们的 功能。正在继续努力确定编码的mRNAs是否 因为核糖体蛋白在哺乳动物细胞中很难翻译 在鸡胚成纤维细胞中进行培养和体内实验。有证据表明 已经累积到表明这是HeLa和3T3的情况 细胞。最后，为了了解胰岛素的作用机制， 该激素对磷脂酰肌醇代谢的影响正在研究中 在鸡胚成纤维细胞中，并且正在比较这些效应 胰岛素和佛波酯在培养的细胞上。(N)

We are continuing our search for monoclonal antibodies that are directed against nuclear antigens whose levels change significantly during the G1 period of primary cultures of chick embryo fibroblasts. We have improved the sensitivity of the ELISA assay. Nevertheless, examination of several thousand hybridoma supernatant fluids has not yet yielded a positive result. As an offshoot of this project, we have begun using monoclonal antibodies to look for differences in the levels of nuclear proteins among the various types of specialized cells that make up the chick embryo kidney. Two very interesting monoclonal antibodies have been found, and efforts are now being made to identify the antigens and to discover their functions. Efforts are continuing to ascertain whether the mRNAs that code for ribosomal proteins are translated with difficulty in mammalian cells in culture and in vivo as they are in chick embryo fibroblasts. Evidence has already been accumulated to show that this is the case for HeLa and 3T3 cells. Finally, to learn about the mechanism of action of insulin, the impact of the hormone on phosphatidylinositol metabolism is being studied in chick embryo fibroblasts, and comparisons are being made of the effects of insulin and phorbol esters on the cultured cells. (N)