MECHANISMS OF CONTROL OF MAMMALIAN CELL MULTIPLICATION

哺乳动物细胞增殖的控制机制

• 批准号: 3163237
• 负责人: IRVING LIEBERMAN
• 金额: $ 13.47万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-06-01 至 1988-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3163237
• 关键词: DNA directed RNA polymerase; RNA methylation; cell age; cell cycle; cell free system; cell fusion; cell growth regulation; cell nucleus; erythrocyte membrane; gel electrophoresis; growth factor; messenger RNA; nucleolus; paper chromatography; ribosomes; transfer RNA

We are continuing our search for monoclonal antibodies that are directed against nuclear antigens whose levels change significantly during the G1 period of primary cultures of chick embryo fibroblasts. We have improved the sensitivity of the ELISA assay. Nevertheless, examination of several thousand hybridoma supernatant fluids has not yet yielded a positive result. As an offshoot of this project, we have begun using monoclonal antibodies to look for differences in the levels of nuclear proteins among the various types of specialized cells that make up the chick embryo kidney. Two very interesting monoclonal antibodies have been found, and efforts are now being made to identify the antigens and to discover their functions. Efforts are continuing to ascertain whether the mRNAs that code for ribosomal proteins are translated with difficulty in mammalian cells in culture and in vivo as they are in chick embryo fibroblasts. Evidence has already been accumulated to show that this is the case for HeLa and 3T3 cells. Finally, to learn about the mechanism of action of insulin, the impact of the hormone on phosphatidylinositol metabolism is being studied in chick embryo fibroblasts, and comparisons are being made of the effects of insulin and phorbol esters on the cultured cells. (N)

我们正在继续寻找定向的单克隆抗体 针对在 G1 期间水平发生显着变化的核抗原 鸡胚成纤维细胞原代培养期。 我们已经改进了 ELISA 测定的灵敏度。 尽管如此，检查了几个 千杂交瘤上清液尚未产生阳性 结果。 作为该项目的一个分支，我们已经开始使用单克隆 抗体来寻找核蛋白水平的差异 构成鸡胚胎的各种类型的特化细胞 肾。 发现了两种非常有趣的单克隆抗体，并且 目前正在努力鉴定抗原并发现其 功能。 正在继续努力确定编码的 mRNA 是否 因为核糖体蛋白在哺乳动物细胞中很难翻译 培养物和体内的鸡胚成纤维细胞。 证据有 已经积累表明 HeLa 和 3T3 就是这种情况 细胞。 最后，为了了解胰岛素的作用机制， 正在研究激素对磷脂酰肌醇代谢的影响 在鸡胚成纤维细胞中，并且正在对效果进行比较 胰岛素和佛波酯对培养细胞的影响。 (N)