Identification of the molecular pathways that drive and steer pseudopods

识别驱动和引导伪足的分子途径

• 批准号: BB/K009699/1
• 负责人: Robert Kay
• 金额: $ 31.78万
• 依托单位: MRC Centre Cambridge
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2013
• 资助国家: 英国
• 起止时间: 2013 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FK009699%2F1
• 关键词: Identification; molecular; pathways; drive; steer

Many cells are able to crawl very slowly over a solid surface or burrow their way through a tissue. Often they are guided by chemicals that attract them and in this way, they can perform a useful purpose. For instance white blood cells are attracted by chemicals inadvertently released bacteria, which they then engulf and destroy. Similarly, amoebae are attracted to bacteria, which they then consume as food. The first step in movement is to make projection - a pseudopod - in the direction of travel. This pseudopod can form anywhere around the cell and is driven out by tiny motor which uses some of the same components as muscle. The position where the pseudopod does form is somehow governed by the concentration of attractive chemical around the cell. The aim of the work proposed here is to learn more about how a pseudopod is produced in the right place so that the cell moves towards an attractive chemical.

许多细胞能够在固体表面缓慢爬行或在组织中钻洞。它们通常受到吸引它们的化学物质的引导，通过这种方式，它们可以发挥有用的作用。例如，白细胞会被无意中释放出来的细菌所吸引，然后它们会吞噬并消灭细菌。同样，变形虫也会被细菌吸引，然后把细菌当作食物吃掉。运动的第一步是在运动方向上做投影——一个伪足。这种假足可以在细胞周围的任何地方形成，并由微型马达驱动，马达使用一些与肌肉相同的成分。假足形成的位置在某种程度上是由细胞周围吸引化学物质的浓度决定的。这里提出的工作的目的是更多地了解假足是如何在正确的地方产生的，以便细胞向有吸引力的化学物质移动。

项目成果

A plasma membrane template for macropinocytic cups

• DOI: 10.7554/elife.20085
• 发表时间: 2016-12-13
• 期刊: ELIFE
• 影响因子: 7.7
• 作者: Veltman, Douwe M.;Williams, Thomas D.;Kay, Robert R.
• 通讯作者: Kay, Robert R.

Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria.

基于细菌选择和生长的盘基网柄菌细胞基因工程。

• DOI: 10.3791/58981
• 发表时间: 2019
• 期刊: JoVE
• 作者: Paschke P

Chemotaxis of a model organism: progress with Dictyostelium.

模型生物的趋化性：盘基网柄菌的进展。

• DOI: 10.1016/j.ceb.2015.06.005
• 发表时间: 2015
• 期刊: Current opinion in cell biology
• 影响因子: 7.5
• 作者: Nichols JM

Drink or drive: competition between macropinocytosis and cell migration.

饮酒或驾驶：巨胞饮作用和细胞迁移之间的竞争。

• DOI: 10.1042/bst20140251
• 发表时间: 2015
• 期刊: Biochemical Society transactions
• 影响因子: 3.9
• 作者: Veltman DM

Neurofibromin controls macropinocytosis and phagocytosis in Dictyostelium.

• DOI: 10.7554/elife.04940
• 发表时间: 2015-03-27
• 期刊: eLife
• 影响因子: 7.7
• 作者: Bloomfield G;Traynor D;Sander SP;Veltman DM;Pachebat JA;Kay RR
• 通讯作者: Kay RR