Sequencing of Epigenetic Marks

表观遗传标记的测序

• 批准号: BB/K010859/1
• 负责人: Sir Shankar Balasubramanian
• 金额: $ 16.52万
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2012
• 资助国家: 英国
• 起止时间: 2012 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FK010859%2F1
• 关键词: Sequencing; Epigenetic; Marks

The ultimate goal is to more accurately sequence the mammalian genome, for the first time identifying both of the modified DNA bases (epigenetic marks) of 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) in addition to the 4 primary bases. This will lead to better knowledge of cell function, have important implications in stem cell research, personalised and regenerative medicines, and show substantial commercial utility. All cells in the body have the same DNA sequence but its interpretation (epigenetics) results in formation of different cell types. 5-mC is a well-known epigenetic mark, but the function of 5-hmC is as yet unknown. Our method specifically locates and quantifies these epigenetic marks together in DNA. We will optimise this method to allow its commercial use more widely on more difficult cell types and the whole human genome. Decoding these epigenetic marks will provide greater understanding of cell regulation and could open up new ways of diagnosing disease.

最终目标是更准确地对哺乳动物基因组进行测序，除了4个主要碱基外，首次鉴定5-甲基胞嘧啶（5 mC）和5-羟甲基胞嘧啶（5 hmC）的修饰DNA碱基（表观遗传标记）。这将导致更好地了解细胞功能，对干细胞研究，个性化和再生药物具有重要意义，并显示出巨大的商业实用性。身体中的所有细胞都具有相同的DNA序列，但其解释（表观遗传学）导致形成不同的细胞类型。5-mC是一个众所周知的表观遗传标记，但5-hmC的功能还不清楚。我们的方法专门定位和量化这些表观遗传标记在DNA中。我们将优化这种方法，使其在更困难的细胞类型和整个人类基因组上更广泛地商业化应用。解码这些表观遗传标记将提供对细胞调节的更好理解，并可能开辟诊断疾病的新方法。

项目成果

In vivo genome-wide profiling reveals a tissue-specific role for 5-formylcytosine.

• DOI: 10.1186/s13059-016-1001-5
• 发表时间: 2016-06-29
• 期刊: Genome biology
• 影响因子: 12.3
• 作者: Iurlaro M;McInroy GR;Burgess HE;Dean W;Raiber EA;Bachman M;Beraldi D;Balasubramanian S;Reik W
• 通讯作者: Reik W

Oxidative bisulfite sequencing of 5-methylcytosine and 5-hydroxymethylcytosine.

• DOI: 10.1038/nprot.2013.115
• 发表时间: 2013-10
• 期刊: Nature protocols
• 影响因子: 14.8