Chemical Mapping of G-Quadruplexes in the Genome

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The DNA found in living cells is the basis for programming the functions associated with life. It has been assumed that all DNA exists primarily as a canonical two stranded double helix, as proposed by Watson and Crick. However, there is mounting evidence to suggest that a four stranded quadruple helix DNA structure, called a quadruplex, may be able to form, and may have a functional significance. This proposal aims to directly explore whether such quadruplex structures exist in cell and to localise where in the ~ 3 Billion bases of DNA, in the human geneome, quadruplexes exist. The outcome(s) will directly address the central hypothesis and may also identify new functional targets for future therapeutics.