权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

ANALYSIS OF THE CELLULAR ONCOGENE PROTO-MYB

细胞癌基因原-MYB 的分析

基本信息

批准号：
3170071
负责人：
DIANA K SHEINESS
金额：
$ 11.33万
依托单位：
ELITECHGROUP, INC.
依托单位国家：
美国
项目类别：
财政年份：
1987
资助国家：
美国
起止时间：
1987-01-03 至 1988-12-31
项目状态：
已结题

项目摘要

A host origin for retroviral oncogenes is implied by the discovery that vertebrate DNA contains highly conserved genes ("proto-oncogenes") that are homologous to retroviral oncogenes. The similarity between viral oncogenes and proto-oncogenes implies a normal role for proto-oncogenes in cell growth and/or differentiation. Our long-term goal is to elucidate the presumptive roles of proto-oncogenes as cancer genes and as regulators of normal cell growth. To that effect, we have conducted studies focused primarily on the expression of proto-myb, a proto-oncogene that corresponds to the transforming gene of the avian myeloblastosis virus. In current studies with mice, we have shown that proto-myb expression is confined exclusively to cells in early stages of differentiation in the hemopoietic lineages and that the highest level of proto-myb mRNA observed was that seen in the thymus. In other experiments, we have ruled out any correlation between expression of several proto-oncogenes and thymic involution. Preliminary results with cultured cells suggest furthermore that proto-myh mRNA levels are not dependent on the proliferative state of the cells. At present, our efforts are directed primarily towards cloning and characterizing the mouse proto-myb gene. By screening a library of mouse DNA in phage lambda, we have obtained clones of genome DNA that evidently contain the 5' end of the proto-myb gene. We have also isolated a cDNA clone containing 2.5 kb of sequence, most of which extends 3' of the cloned genome DNA. By restriction mapping, we have estimated a size of 40 Kb for the mouse proto-myb gene. Selected portions of the cloned DNAs are currently being sequenced. Additional efforts are underway to clone the remainder of the proto-myb gene and to study proto-myb expression thymocytes exposed to mitogens and hormones. (P)

逆转录病毒癌基因的宿主起源是由以下发现暗示的，脊椎动物DNA含有高度保守的基因（“原癌基因”），与逆转录病毒癌基因同源。病毒癌基因之间的相似性而原癌基因则意味着原癌基因在细胞中的正常作用生长和/或分化。我们的长期目标是阐明原癌基因作为癌基因和作为调节因子的假定作用正常的细胞生长。为此，我们进行了研究，主要依赖于原myb的表达，禽成髓细胞瘤病毒的转化基因在目前对小鼠的研究中，我们已经表明，仅限于细胞在分化的早期阶段，造血谱系和最高水平的原myb mRNA观察就是胸腺中的那个在其他实验中，我们已经排除了任何几种原癌基因的表达与胸腺对合培养细胞的初步结果表明，原myh mRNA水平不依赖于细胞的增殖状态，细胞。目前，我们的努力主要针对克隆，小鼠原myb基因的特征。通过筛选小鼠文库在λ噬菌体中，我们已经获得了基因组DNA的克隆，含有原myb基因的5 ′端。我们还分离了一个cDNA 含有2.5 kb序列的克隆，其中大部分延伸到克隆的3'端基因组DNA 通过限制性酶切作图，我们估计了小鼠原myb基因。克隆DNA的选定部分是目前正在测序中。目前正在进行更多的努力，原myb基因的剩余部分，并研究原myb基因的表达胸腺细胞暴露于有丝分裂原和激素。（P）