OLIGONUCLEOTIDES FOR DIAGNOSIS OF VAGINITIS

拟议的工作将导致一个简单易用和廉价的 原型测试套件，用于快速准确地区分 主要病原体负责阴道炎。 阴道炎是最常见的 女性寻求医疗照顾的常见情况。 这个套件将 在医生办公室、临床和实验室环境中使用。为 在该测试中，将开发一组合成寡核苷酸， 特异性检测释放的丰富的16 S和23 S核糖体RNA 目标微生物。这将通过三明治来完成 生物体特异性寡核苷酸将被 共价偶联到固体支持物上，并允许与 裂解-杂交试剂。 所得到的捕获的rRNA将被 通过加入第二组配核苷酸来检测， 与rRNA中存在的保守序列杂交。 该第二 一组DNA探针将与一个高灵敏度的比色仪结合， 信号产生系统 最后，由此开发的原型套件将 使用临床样本进行评估。

The proposed work will result in a simple-to-use and inexpensive prototype test kit for the rapid and accurate differentiation of the major pathogens responsible for vaginitis. Vaginitis is among the most common conditions for which women seek medical attention. This kit will be used in the doctor's office, clinical and laboratory settings. For this test, a panel of synthetic oligonucleotides will be developed that specifically detects the abundant 16S and 23S ribosomal RNAs released from target microorganisms. This will be accomplished using a sandwich assay format in which the organism-specific oligonucleotides will be covalently coupled to a solid support and allowed to hybridized with lysis-hybridization reagent. The resulting captured rRNAs will be detected by the addition of a second set of ligonucleotides that will hybridize with the conserved sequences present in the rRNA. This second set of DNA probes will be coupled with a highly sensitive clorimetric signal production system. Finally, the prototype kit thus developed will be evaluated using clinical specimens.