OLIGONUCLEOTIDES FOR DIAGNOSIS OF VAGINITIS

拟议的工作将产生一种简单易用且成本低廉的 用于快速准确地区分 阴道炎的主要病原体。阴道炎是最常见的 妇女寻求医疗救治的常见情况。这个工具包将会 适用于医生办公室、临床和实验室环境。为 在这项测试中，将开发一组合成寡核苷酸 特异性检测释放的丰富的16S和23S核糖体RNA 来自目标微生物的。这将通过使用三明治来实现 生物体特异性寡核苷酸将采用的检测格式 共价偶联到固体载体上，并允许与 裂解杂交试剂。最终捕获的rRNA将是 通过添加第二组连接核苷酸来检测，这将 与rRNA中存在的保守序列杂交。这一秒 一组DNA探针将与高灵敏的示差扫描仪耦合 信号产生系统。最后，这样开发的原型套件将 使用临床标本进行评估。

The proposed work will result in a simple-to-use and inexpensive prototype test kit for the rapid and accurate differentiation of the major pathogens responsible for vaginitis. Vaginitis is among the most common conditions for which women seek medical attention. This kit will be used in the doctor's office, clinical and laboratory settings. For this test, a panel of synthetic oligonucleotides will be developed that specifically detects the abundant 16S and 23S ribosomal RNAs released from target microorganisms. This will be accomplished using a sandwich assay format in which the organism-specific oligonucleotides will be covalently coupled to a solid support and allowed to hybridized with lysis-hybridization reagent. The resulting captured rRNAs will be detected by the addition of a second set of ligonucleotides that will hybridize with the conserved sequences present in the rRNA. This second set of DNA probes will be coupled with a highly sensitive clorimetric signal production system. Finally, the prototype kit thus developed will be evaluated using clinical specimens.