DNA PROBE TEST TO DETECT ORAL CANDIDA IN AIDS PATIENTS

DNA 探针检测艾滋病患者口腔念珠菌

• 批准号: 3489168
• 负责人: DIANA K SHEINESS
• 金额: $ 5万
• 依托单位: ELITECHGROUP, INC.
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-30 至 1991-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3489168
• 关键词: AIDS; Candida albicans; HIV infections; diagnosis design /evaluation; human subject; immunosuppression; medical complication; microorganism classification; nucleic acid hybridization; nucleic acid probes; nucleic acid sequence; oligonucleotides; opportunistic infections; oral bacteria; rapid diagnosis

The work proposed here will provide the foundation for a rapid, inexpensive, non-culture based test for the quantitative detection of oral C albicans in HIV-positive patients. The primary experimental objective will be to create a set of short synthetic oligodeoxynucleotide probes that will hybridize specifically with the 18S rRNA of Candida albicans. Specificity will be verified by hybridization of each probe to slot blot filters containing RNAs from > 100 microorganisms that are found frequently in the oropharynx, skin or urogenital region. Furthermore, the probes wig be used to measure levels of C albicans in mouth rinses from immunocompromised patients. Probe assays will be compared with conventional diagnostic evaluations of candidiasis in these same patients. Results of this comparison will establish the feasibility of using probes as an alternative to culture for diagnosing candidiasis. If this approach proves to be efficacious, these probes win be used during Phase H to develop a rapid, non-radioactive assay for detecting and monitoring C albicans in persons at high risk for developing acquired immunodeficiency syndrome (AIDS). The final product will be a quantitative test for the doctor's office that can be performed in less than one hour at room temperature and that can provide an alternative for the much lengthier procedure of culturing.

这里提出的工作将为快速、 用于定量检测口腔细菌的廉价、非培养测试 HIV 阳性患者中的白色念珠菌。主要实验目的 将创建一组短的合成寡脱氧核苷酸探针 将与白色念珠菌的 18S rRNA 特异性杂交。 特异性将通过每个探针与狭缝印迹的杂交来验证 含有来自超过 100 种常见微生物的 RNA 的过滤器 在口咽、皮肤或泌尿生殖区域。此外，探针假发 用于测量漱口水中白色念珠菌的含量 免疫功能低下的患者。探针检测将与传统检测进行比较 对这些相同患者的念珠菌病进行诊断评估。结果 这种比较将确定使用探针作为 替代培养来诊断念珠菌病。如果这个方法证明 为了有效，这些探针将在 H 阶段使用，以开发 用于检测和监测白色念珠菌的快速、非放射性测定 患获得性免疫缺陷综合症的高风险人群 （艾滋病）。最终产品将作为医生的定量测试 可以在室温下在一小时内完成的办公室 这可以为更长的程序提供替代方案 培养。