SOMATIC CELL GENETICS OF T-CELL DIFFERENTIATION ANTIGENS

我们将使用体细胞遗传学技术来分析Lyt2的作用 (T8)、L3T4(T4)和Ly5(T200)在细胞功能和分化中的作用 属于T血统。我们已经开发出产生体细胞变体的方法 克隆的正常细胞和转化的细胞，我们建议使用这些 选择“正常”T细胞或T细胞前体细胞系的技术 其缺少或表达改变形式的表面标记，该表面标记 通常作为分化抗原或细胞谱系标志。我们 也会产生异常正常表达标志物的细胞系 存在于谱系的其他细胞中或源自其他物种。 我们将克隆这些细胞，在大规模培养中培养它们，并研究它们的 抗原结构、遗传组织、生物行为和 函数属性，将它们与它们的父行和 免疫选择的反转变种。具体地说，我们计划准备一种 过早表达Lyt2(或OKT8)和 L3T4(或OKT4)，并研究它们随后的分化。我们还将 制备缺乏L3T4或表达L3T4的辅助性T细胞株的变体 人类等效抗原(OKT4)或表达该抗原 与抑制子表型(OKT8)相关。

We shall use somatic cell genetic techniques to analyze the role of Lyt2 (T8), L3T4 (T4) and Ly5 (T200) in the function and differentiation of cells of the T lineage. We have developed methods of producing somatic variants of cloned normal and transformed cells, and we propose to use these techniques to select cell lines of "normal" T cells or T-cell precursors which either lack or express an altered form of a surface marker which normally serves as a differentiation antigen or cell lineage marker. We shall also produce cell lines which egregiously express markers normally present in other cells of the lineage or are derived from other species. We shall clone these cells, grow them in mass culture and study their antigenic structure, genetic organization, biological behavior and functional properties, comparing them with both their parent line and with immunoselected revertants. Specifically, we plan to prepare variants of a cultured prothymocyte line which prematurely express Lyt2 (or OKT8) and L3T4 (or OKT4) and study their subsequent differentiation. We will also prepare variants of helper T cell lines which either lack L3T4, or express either the human equivalent antigen (OKT4) or express the antigen associated with the suppressor phenotype (OKT8).