CORE--FLOW CYTOMETRY FACILITY

流式细胞术单位是卡普兰的跨部门资源 纽约大学医学中心的综合癌症中心，其目标是 提供最先进的流程技术服务和培训 给癌症中心的成员这些服务旨在 促进成员的研究，教育他们关于 流动技术和培训提供的技术可能性 和博士后学生在使用这项技术。 该设施操作三种仪器：库尔特EPICS精英，一个Becton Dickinson FACStar Plus和Becton Dickinson FACScan。这些都被安置在 在Skirball研究所三楼的新宿舍里流动 流式细胞仪实验室提供灵敏的多参数细胞标记物 分析;脱氧核糖核酸含量的定量研究;动力学测量 细胞内钙和pH;以及膜流动性的测量 作为包被有细胞的颗粒的相互作用的定量 粘附分子分析质量控制由每日 标准化的仪器与参考珠和由 常规纳入参比样品。 该实验室还提供细胞分选能力。样品 准备工作是设施使用者的责任。细胞 选择可以在多达五个参数上执行， 7,000个细胞/秒。一种单细胞沉积装置（AutoClone）， 保持无菌状态并允许使用器械 用于克隆细胞。 该中心的所有成员都可以先到先得地使用该设施， 先到先得。癌症中心成员优先于非 会员优先于非会员。该设施由一个 使用费、机构支持和癌症中心相结合 核心补助

The Flow Cytometry Unit is an interdepartmental resource of the Kaplan Comprehensive Cancer Center at the NYU Medical Center whose objective is to make available state-of-the-art flow technology service and training to the members of the Cancer Center. These services are designed to facilitate the research of the members, educate them about the technological possibilities offered by flow techniques and training pre- and post-doctoral students in the use of this technology. The facility operates three instruments: a Coulter EPICS Elite, a Becton Dickinson FACStar Plus and a Becton Dickinson FACScan. These are housed in new quarters on the 3rd floor of the Skirball Institute. The Flow Cytometry ore Laboratory provides sensitive multi-parameter cell marker analysis; quantitative studies of DNA content; kinetic measurements of intracellular Calcium and pH; measurement of membrane fluidity as well as quantitation of the interactions of particles coated with cell adhesion molecules. Analytical quality control is maintained by daily standardization of the instruments with reference beads and by the routine inclusion of reference samples. The laboratory also provides cell-sorting capabilities. Sample preparation is the responsibility of the users of the facility. Cell selection can be performed on up to five parameters at rates exceeding 7,000 cells/second. A single cell deposition device (AutoClone) is available that maintains sterility and permits the instrument to be used for cloning cells. All members of the Center have access to the facility on a first come, first served basis. Cancer Center members have priority over non members have priority over non members. The facility is supported by a combination of user fees, institutional support and the Cancer Center Core Grant.