CORE--FLOW CYTOMETRY FACILITY

核心——流式细胞仪

• 批准号: 6101771
• 负责人: ROSS S BASCH
• 金额: $ 13.42万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-10 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6101771
• 关键词: DNA; acid base balance; biomedical facility; calcium flux; cell adhesion molecules; clinical research; flow cytometry; human tissue; membrane activity; neoplasm /cancer; neoplastic cell

The Flow Cytometry Unit is an interdepartmental resource of the Kaplan Comprehensive Cancer Center at the NYU Medical Center whose objective is to make available state-of-the-art flow technology service and training to the members of the Cancer Center. These services are designed to facilitate the research of the members, educate them about the technological possibilities offered by flow techniques and training pre- and post-doctoral students in the use of this technology. The facility operates three instruments: a Coulter EPICS Elite, a Becton Dickinson FACStar Plus and a Becton Dickinson FACScan. These are housed in new quarters on the 3rd floor of the Skirball Institute. The Flow Cytometry ore Laboratory provides sensitive multi-parameter cell marker analysis; quantitative studies of DNA content; kinetic measurements of intracellular Calcium and pH; measurement of membrane fluidity as well as quantitation of the interactions of particles coated with cell adhesion molecules. Analytical quality control is maintained by daily standardization of the instruments with reference beads and by the routine inclusion of reference samples. The laboratory also provides cell-sorting capabilities. Sample preparation is the responsibility of the users of the facility. Cell selection can be performed on up to five parameters at rates exceeding 7,000 cells/second. A single cell deposition device (AutoClone) is available that maintains sterility and permits the instrument to be used for cloning cells. All members of the Center have access to the facility on a first come, first served basis. Cancer Center members have priority over non members have priority over non members. The facility is supported by a combination of user fees, institutional support and the Cancer Center Core Grant.

流式细胞术单位是卡普兰的一个部门间资源。 纽约大学医学中心的综合癌症中心，其目标是 提供最先进的流程技术服务和培训 致癌症中心的成员。这些服务旨在 促进会员的研究，教育他们关于 流动技术和培训前提供的技术可能性 以及博士后对这项技术的使用。 该设施运营着三台仪器：一台库尔特史诗精英，一台贝顿 Dickinson FACStar Plus和Becton Dickinson FACScan。这些都是住房 在Skirball学院3楼的新宿舍里。流动 OR实验室提供灵敏的多参数细胞标记物 分析.DNA含量的定量研究.动力学测量 细胞内钙和pH；以及膜流动性的测量 作为包被细胞的颗粒相互作用的定量 黏附分子。分析质量控制由日常维护 使用参考珠子和通过 参考样品的常规包含。 该实验室还提供细胞分选能力。样本 准备工作是设施使用者的责任。细胞 最多可以对五个参数执行选择，选择的速率超过 7000个细胞/秒。单细胞沉积设备(AutoClone)是 可保持无菌状态并允许使用该仪器 用于克隆细胞。 该中心的所有成员都可以在第一时间进入该设施， 先到先得。癌症中心会员优先于非会员 会员优先于非会员。该设施由一个 使用费、机构支持和癌症中心的结合 科尔·格兰特。