HUMAN MYELOMA CELL LINE

人骨髓瘤细胞系

• 批准号: 3171305
• 负责人: GEORGE T BRYAN
• 金额: $ 11.97万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-09-30 至 1987-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3171305
• 关键词: B lymphocyte; antibody formation; antibody specificity; antitumor antibody; cell bank /registry; cell differentiation; cell fusion; cell mediated cytotoxicity; gel filtration chromatography; human subject; hybridomas; ion exchange chromatography; leukocyte activation /transformation; monoclonal antibody; multiple myeloma; neoplasm /cancer diagnosis; neoplasm /cancer immunology; neoplasm /cancer therapy; radionuclides; scintillation counter; tissue /cell culture; tumor antigens

The goal is to develop a long-term human myeloma cell line that will provide valuable substrates for subcategorizing B-cell malignancies, for generating human-derived monoclonal antibodies that may become major diagnostic and/or therapeutic tools, and for understanding the normal and abnormal process of human B-cell maturation. A long-term plasma cell line, derived from a patient with multiple myeloma, has been characterized, subcloned, and used as antigen in the genesis of mouse monoclonal antibodies directed against human plasma cells and/or human myeloma cells. The role of growth and differentiation factors in the maintenance of the long-term line has been explored and attempts made to utilize such factors in establishing growth of other human myeloma cells. Eight additional human plasma cell lines have been developed one of which is a nonsecretor. These lines grow in nude mice and secrete immunoglobulin. Some successful human-human hybridizations have occurred. (2)

其目标是开发一种长期的人类骨髓瘤细胞系，该细胞系将 为细分B细胞恶性肿瘤提供有价值的底物， 产生人类来源的单抗，可能成为主要的 诊断和/或治疗工具，并用于了解正常和 人类B细胞成熟过程异常。 一种来自多发性骨髓瘤患者的长期浆细胞系， 已被鉴定、亚克隆并作为抗原用于病毒的起源 小鼠抗人浆细胞和/或 人骨髓瘤细胞。生长分化因子在肿瘤生长和分化中的作用 对长期线路的维护进行了探索，并尝试 利用这些因素来建立其他人类骨髓瘤细胞的生长。 另外开发了八种人类浆细胞系，其中一种 是个不为人知的人。这些细胞系在裸鼠体内生长并分泌 免疫球蛋白。已经发生了一些成功的人与人的杂交。 (2)

项目成果

Spurious E rosette formation in B cell chronic lymphocytic leukemia due to monoclonal anti-sheep RBC antibody.

单克隆抗羊红细胞抗体导致 B 细胞慢性淋巴细胞白血病中虚假 E 玫瑰花结的形成。

• 发表时间: 1985
• 期刊: Blood
• 影响因子: 20.3
• 作者: Mills,LE;O'Donnell,JF;Guyre,PM;LeMarbre,PJ;Miller,JD;Bernier,GM
• 通讯作者: Bernier,GM

Monoclonal antibodies--of mice and men.

小鼠和人的单克隆抗体。

• 发表时间: 1986
• 期刊: Transactions of the American Clinical and Climatological Association
• 作者: Bernier,GM;Miller,JD;Mills,LE
• 通讯作者: Mills,LE