HUMAN CELL SURFACE ANTIGENS: TRANSFERRIN RECEPTORS

人类细胞表面抗原：转铁蛋白受体

• 批准号: 3172606
• 负责人: IAN S TROWBRIDGE
• 金额: $ 11.64万
• 依托单位: SALK INSTITUTE FOR BIOLOGICAL STUDIES
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-06-01 至 1986-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3172606
• 关键词: chemical structure function; gene expression; genetic manipulation; genetic regulation; human tissue; monoclonal antibody; mutant; neoplasm /cancer immunology; neoplastic growth; surface antigens; tissue /cell culture; transferrin

Monoclonal antibodies have been used in earlier studies in this laboratory to identify and characterize the transferrin receptors of human and murine cells. Transferrin receptors are associated with active cell growth and are selectively expressed on some tumors in vivo. Monoclonal antibodies have been obtained that block receptor function and inhibit tumor cell growth in vitro. This work is being developed in two directions. The human transferrin receptor gene has been isolated and can be expressed in the appropriate cell type. Efforts are underway to use oligonucleotide site-directed mutagenesis E to study the structure-function relationship of the human transferrin receptor. The other aspect of the project is to investigate the effects of monoclonal antibodies that block transferrin receptor function on the growth of tumor cells in vivo. In the past year, it has been shown that a rat IgM monoclonal antibody against the murine transferrin receptor can inhibit the growth of a syngeneic leukemia, SL-2, in AKR/J mice. Both inhibition of growth of the primary subcutaneous tumor and prolonged survival of tumor-bearing mice were demonstrated. There was no evidence of toxicity associated with administration of the antibody. The distribution of transferrin receptors on murine hematopoietic stem cells has also been investigated. (CS)

单克隆抗体已用于本实验室早期的研究中 鉴定和表征人和鼠的转铁蛋白受体， 细胞 转铁蛋白受体与活跃的细胞生长有关， 在体内的某些肿瘤上选择性表达。 单克隆抗体 已经获得了阻断受体功能并抑制肿瘤细胞 体外生长这项工作正朝着两个方向发展。 人类 转铁蛋白受体基因已被分离，并可在 合适的细胞类型。 正在努力使用寡核苷酸 定点突变E，以研究结构-功能关系 人类转铁蛋白受体 该项目的另一方面是 研究阻断转铁蛋白的单克隆抗体的作用 受体功能对体内肿瘤细胞生长的影响。 在过去的一年里， 已经表明，大鼠抗鼠IgM单克隆抗体 转铁蛋白受体可以抑制同系白血病SL-2的生长， AKR/J小鼠。 抑制原发性皮下肿瘤的生长 并延长了荷瘤小鼠的存活时间。 有 没有与抗体给药相关的毒性证据。 转铁蛋白受体在小鼠造血干细胞上的分布 细胞也进行了研究。 （中文）