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BLOOD GROUP RELATED ANTIGENS IN HUMAN BLADDER CANCER

人类膀胱癌中的血型相关抗原

基本信息

批准号：
3171815
负责人：
JOHN S COON
金额：
$ 11.97万
依托单位：
RUSH UNIVERSITY MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
1983
资助国家：
美国
起止时间：
1983-03-01 至 1986-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3171815
关键词：
bioassay bladder neoplasm blood group antigens carcinoma cell population study flow cytometry human tissue immunoelectron microscopy immunogenetics metastasis neoplasm /cancer genetics neoplasm /cancer invasiveness neoplasm /cancer relapse /recurrence neoplastic cell surface antigens tissue /cell culture

项目摘要

Numerous retrospective immunohistological studies have shown a correlation between deletion of normal epithelial ABH "blood group" antigens from noninvasive urinary bladder carcinomas and development of recurrent carcinomas with deep muscle invasion. We have recently shown that detection of the normally cryptic T (Thomsen-Friedenreich) blood group related antigen in tumors provides additional prognostic information. However, nothing is known of the biological basis for the correlations between the profile of blood group related antigens on tumor cell surfaces and malignant phenotype. Also, it is unclear whether purely qualitative antigen testing as currently performed will be clinically useful for assessing prognosis and therapy for the individual patient. Because of the inherent limitations of retrospective, immunohistologic studies, we have focused our attention on antigenically defined cell lines. Our overall objective is to test the hopothesis that the correlations observed in retrospective studies may reflect a quantitative link between expression of blood group related cell surface antigens and malignant phenotype (invasion, metastasis) at the level of defined tumor cells populations. We plan to establish by flow cytometry from continuous human urinary bladder carcinoma cell lines, sublines with defined profiles of blood group related cell surface antigens. We will then correlate antigen expression (quantitative cytometry, immunoelectron microscopy) with quantifiable parameters of the malignant phenotype of each subline; a) tumorigenicity; b) growth in semisolid media; c) hyperploidy; d) invasion (bone invasion assay); e) metastasis (ling colony assay).

许多回顾性免疫组织学研究表明，正常上皮ABH“血型”抗原的缺失非侵袭性膀胱癌和复发性膀胱癌的发展有深部肌肉浸润的癌。我们最近的研究表明正常隐匿T（B-F）血型的检测肿瘤中的相关抗原提供了额外的预后信息。然而，对于这种相关性的生物学基础，肿瘤细胞表面血型相关抗原的分布和恶性表型。此外，尚不清楚是否纯粹定性目前进行的抗原检测将在临床上用于评估个体患者的预后和治疗。因为回顾性免疫组织学研究的固有局限性，将我们的注意力集中在抗原性确定的细胞系上。我们的整体目的是测试观察到的相关性的假设回顾性研究可能反映了表达之间的定量联系，血型相关细胞表面抗原与恶性表型在确定的肿瘤细胞群体的水平上检测肿瘤的侵袭（侵袭、转移）。我们计划通过流式细胞术从连续的人膀胱中建立癌细胞系，具有确定的血型相关谱的亚系细胞表面抗原然后我们将抗原表达（定量细胞术，免疫电镜）每个亚系的恶性表型参数; a）致瘤性; B）在半固体培养基中生长; c）超倍性; d）侵袭（骨侵袭 e）转移（Ling集落测定）。