IN VITRO INDUCTION OF NK CYTOTOXICITY

人类混合淋巴细胞培养(MLC)可用于产生自然杀伤细胞 (NK)或NK样细胞毒作用。这种细胞毒性不是针对 同种异体抗原，可由NK不活跃的前体细胞产生。这 研究涉及对这一现象的详细研究，以便确定 T细胞功能与NK细胞杀伤活性的关系实验性的 该研究的目标包括研究MLC诱导的NK细胞的特异性 通过对一大批靶点的反应性分析来实现细胞毒性 细胞。将特别强调针对原生生物的反应性 活检获得肿瘤细胞。MLC诱导的NK细胞的表型将是 研究目的是为了确定这些细胞与人类T细胞的关系 细胞。产生这种反应所需的前体细胞的性质 将从外周血液和其他淋巴组织中进行检查 胸腺等隔室。初步数据表明，细胞毒性 人胸腺细胞可通过以下途径产生具有NK活性的细胞 如果存在白介素2(IL-2)，则为同种异体刺激。这是一款极好的 研究NK细胞与T细胞关系的模型。监管 细胞对NK细胞的表达和诱导杀伤作用将进一步 调查过了。我们的结果表明，细胞能够抑制 NK效应器功能可以在体外产生。这些研究是专门设计的 为了更全面地了解 NK细胞毒性的产生及其与T细胞功能的关系， 以及它们在人类癌症中的可能意义。(磅)

Human mixed lymphocyte culture (MLC) can be used to generate natural killer (NK) or NK-like cytotoxicity. This cytotoxicity is not directed against alloantigens and can be generated from NK-inactive precursor cells. This research involves a detailed study of this phenomenon in order to determine the relationship of T-cell function to NK cytotoxicity. The experimental goals of the research include studies on the specificity of MLC-induced NK cytotoxicity by an analysis of reactivity against a large panel of target cells. Special emphasis will be given to reactivity against autochthonous biopsy obtained tumor cells. The phenotype of MLC-induced NK cells will be studied in order to determine the relationship of these cells to human T cells. The nature of the precursor cells required to generate this response will be examined both from the peripheral blood and from other lymphoid compartments such as the thymus. Preliminary data indicate that cytotoxic cells with NK activity can be generated from human thymocytes by allostimulation if interleukin-2 (IL-2) is present. This is an excellent model for studying the relationship of NK cells to T cells. Regulatory cells for the expression and induction of NK cytotoxicity will be further investigated. Our results indicate that cells capable of suppressing NK-effector function can be generated in vitro. These studies are designed to provide a more comprehensive understanding of the cell types involved in the generation of NK cytotoxicity, their relationship to T-cell functions, and their possible significance in human cancer. (LB)