CELL CYCLE SPECIFIC CONTROL OF CELLULAR DIFFERENTIATION

这些研究调查了细胞分化的调节。这个 具体的主要目标是确定：(1)前置量 分化所需的增殖；(2)细胞周期特异性 调节分化的细胞过程；以及(3)无论是中枢还是 不同的细胞调节过程(ES)影响不同的过程 前体细胞的分化。要使用的蜂窝系统是 人早幼粒白血病细胞株HL-60。受制于不同 诱导剂：HL-60向髓系或单核细胞方向分化 小路。细胞周期动力学和分化动力学 不同诱导剂的影响将使用多参数流动来确定 细胞学分析。调控过程的细胞周期特异性 将这一前体细胞转化为髓系或单核细胞途径 分化将使用同步细胞来确定。初级阶段 涉及的科学问题是：(1)有多少增殖活动 在细胞分化之前；(2)在细胞分化之前 增殖细胞周期与细胞周期调控有关 影响分化；以及(3)相同或不同 细胞调控过程(ES)控制着不同方向的分化 小路？这些研究旨在了解细胞调节 辨证机制与病理特别相关。 血液肿瘤。细胞缺陷与白血病发生的关系 可归因于病理分化过程。这些研究 应该增加对这种病理的理解，并有望促进 洞察更有效的临床管理的新方法 疾病。与此相关的是利用这些研究 调节细胞分化的物质。了解蜂窝网络 对这些药剂的反应可能导致使用这些或 相关化合物。(N)

These studies investigate the regulation of cellular differentiation. The specific primary objectives are to determine: (1)\the amount of anteceding proliferation needed for differentiation; (2)\the cell cycle specificity of cellular processes regulating differentiation; and (3)\whether a central or diverse cellular regulatory process(es) effects different courses of differentiation in a precursor cell. The cell system to be used is the HL-60 human promyelocytic leukemia cell line. Subject to different inducers, HL-60 differentiates along either the myeloid or monocytic pathways. The cell-cycle kinetics and the kinetics of differentiation effected by different inducers will be determined using multiparameter flow cytometric analysis. The cell-cycle specificity of regulatory processes committing this precursor cell to myeloid or monocytic pathways of differentiation will be determined using synchronized cells. The primary scientific questions addressed are: (1)\how much proliferative activity precedes cellular differentiation; (2)\what cellular processes in the proliferative cell cycle are implicated with a regulatory role for effecting differentiation; and (3)\does the same or different cellular-regulatory process(es) govern differentiation along different pathways? These studies directed at understanding the cellular-regulatory mechanism of differentiation are of particular relevance to the pathology of hematological neoplasms. The cellular defect seminal to leukemogenesis is attributable to a pathological differentiative process. The studies should increase the understanding of that pathology and hopefully promote insight into new means of more effective clinical management of such diseases. Of relevance to this is the utilization of these studies of agents which modulate cellular differentiation. Understanding the cellular response to such agents may lead to treatment protocols utilizing these or related compounds. (N)