Fluorescence activated cell sorter

描述(由申请人提供)：这项提案寻求支持在康奈尔大学获得一个荧光激活的细胞分选器，因为那里不再有可行的细胞分选器。该仪器将支持目前由22名教职员工组成的用户组，他们拥有32项NIH研究拨款，外加几笔NSF和USDA/NRI拨款。他们还有3笔NIH(T32)培训补助金。因此，该仪器将满足NIH赞助的这些研究项目中对细胞学的需求，提高正在进行的研究的生产率，并扩大培训计划，然后培训计划可以包括细胞学。这22名教员将在广泛的研究中使用细胞学。这些包括细胞周期调节、细胞因子和激素的信号传递、细胞对感染的反应、钙动员、细胞凋亡、白细胞分类、线粒体功能、细胞分化、异位基因表达和转录调节。流式细胞仪设备将由Andrew Yen领导。当他还是斯隆-凯特琳研究所的研究生时，他就有了做流式细胞术的经验，当时他的导师的实验室获得了第一台商用机器(纽约州毛帕克的生物物理学)。在流式细胞仪的应用方面，他仍然站在替补席上。一名经验丰富的技术人员会做日常运行，导演会和用户一起做故障排除和实验设计，以及数据解释。组织结构将由一名主任和一个指导委员会组成，该委员会由用户社区的6名轮值成员和研究和研究生教育主任组成。将收取使用费，该设施的会计支持将由部门负责。生物医学科学系。由两门现有课程“细胞生理学和基因组学实验室”和“方法迷你课程”的讲座和实验室组成的教育方案以及一年一度的研究研讨会将促进康奈尔大学社区在流式细胞术应用方面的教育。流式细胞仪的资金还将赞助一次定期的用户会议。这些课程、计划和用户会议的秘书支持，包括公告、技术公告和课程材料，将由生物医学科学提供。机构对流式细胞仪的持续承诺将在头三年提供15万美元，并在此后的仪器寿命内提供一份有保证的服务合同。所要求的仪器是一台达科细胞化学基础MoFlo外加一台UV-Vis、Vis和HeNe激光器，以提供独立的UV、VIS/488和红色激发。

DESCRIPTION (provided by applicant): This proposal seeks support to acquire a fluorescence activated cell sorter at Cornell University where there is no longer a viable cell sorter. The instrument will support a user group currently consisting of 22 faculty who have 32 NIH research grants plus several NSF and USDA/NRI grants. They also have 3 NIH (T32) training grants. The instrument will thus fulfill needs for cytometry in these NIH sponsored research programs, enhancing productivity of ongoing research and augmenting the training programs, which can then include cytometry. The 22 faculty will use cytometry in a wide range of studies. These include cell cycle regulation, signaling by cytokines and hormones, cellular response to infection, calcium mobilization, apoptosis, leukocyte typing, mitochondrial function, cell differentiation, ectopic gene expression, and transcriptional regulation. The flow cytometry facility will be directed by Andrew Yen. He has had experience doing flow cytometry since he was a graduate student at the Sloan- Kettering Institute when the first commercial machines (BioPhysics, Mahopac, NY) were acquired in the laboratory of his mentors. He is still at the bench in flow cytometric applications. An experienced technician will do day-to-day running, and the director will do trouble shooting and experimental design, as well as data interpretation, with users. The organizational structure will consist of a director and a guidance committee made of 6 rotating members of the user community and the Director of Research and Graduate Education. User fees will be charged and accounting support for the facility will be by the Dept. of Biomedical Sciences. An educational program consisting of lectures and laboratories in two existing courses, "Cell Physiology and Genomics Laboratory" and "Methods Minicourse" and an annual research symposium will promote education of the Cornell community in applications of flow cytometry. The flow cytometry funding will also sponsor a regular users meeting. Secretarial support for these courses, the program and the user meetings, including announcements, technical bulletins, and course materials, will be provided by Biomedical Sciences. Continuing institutional commitment to flow cytometry will be provided as $150,000 over the first three years and a guaranteed service contract thereafter for the life of the instrument. The instrument requested is a Dako-Cytomation basic MoFlo plus a UV-vis, vis, and HeNe laser to provide independent UV, vis/488, and red excitation.