Fluorescence activated cell sorter

描述（由申请人提供）：本提案寻求在康奈尔大学获得荧光激活细胞分选器的支持，因为那里不再有可行的细胞分选器。该仪器将支持一个用户组，目前由22名教师组成，他们拥有32项NIH研究资助，以及若干项NSF和USDA/NRI资助。他们还获得了3项NIH （T32）培训资助。因此，该仪器将满足这些NIH资助的研究项目对细胞术的需求，提高正在进行的研究的生产力，并增加培训计划，其中可以包括细胞术。22名教员将在广泛的研究中使用细胞术。这些包括细胞周期调节、细胞因子和激素的信号传导、细胞对感染的反应、钙动员、凋亡、白细胞分型、线粒体功能、细胞分化、异位基因表达和转录调节。流式细胞仪设备将由Andrew Yen负责。当他还是斯隆-凯特琳研究所的研究生时，第一台商用机器（生物物理，Mahopac， NY）在他导师的实验室获得时，他就有了做流式细胞术的经验。他仍在流式细胞术应用方面处于初级阶段。一位经验丰富的技术人员将负责日常运行，主管将与用户一起进行故障排除和实验设计，以及数据解释。组织结构将包括一名主任和一个由6名用户社区轮流成员组成的指导委员会，以及研究和研究生教育主任。该设施的使用费将由生物医学科学系收取，会计支持将由生物医学科学系提供。在现有的两门课程“细胞生理学和基因组学实验室”和“方法迷你课程”中，一个由讲座和实验室组成的教育计划和一个年度研究研讨会将促进康奈尔社区在流式细胞术应用方面的教育。流式细胞术基金还将赞助定期的用户会议。生物医学科学部将为这些课程、计划和用户会议提供秘书支持，包括公告、技术公报和课程材料。将在头三年继续为流式细胞术提供15万美元的机构承诺，并在此后的仪器寿命期间提供保证服务合同。要求的仪器是Dako-Cytomation基础MoFlo加上UV-vis， vis和HeNe激光器，以提供独立的UV， vis/488和红色激发。

DESCRIPTION (provided by applicant): This proposal seeks support to acquire a fluorescence activated cell sorter at Cornell University where there is no longer a viable cell sorter. The instrument will support a user group currently consisting of 22 faculty who have 32 NIH research grants plus several NSF and USDA/NRI grants. They also have 3 NIH (T32) training grants. The instrument will thus fulfill needs for cytometry in these NIH sponsored research programs, enhancing productivity of ongoing research and augmenting the training programs, which can then include cytometry. The 22 faculty will use cytometry in a wide range of studies. These include cell cycle regulation, signaling by cytokines and hormones, cellular response to infection, calcium mobilization, apoptosis, leukocyte typing, mitochondrial function, cell differentiation, ectopic gene expression, and transcriptional regulation. The flow cytometry facility will be directed by Andrew Yen. He has had experience doing flow cytometry since he was a graduate student at the Sloan- Kettering Institute when the first commercial machines (BioPhysics, Mahopac, NY) were acquired in the laboratory of his mentors. He is still at the bench in flow cytometric applications. An experienced technician will do day-to-day running, and the director will do trouble shooting and experimental design, as well as data interpretation, with users. The organizational structure will consist of a director and a guidance committee made of 6 rotating members of the user community and the Director of Research and Graduate Education. User fees will be charged and accounting support for the facility will be by the Dept. of Biomedical Sciences. An educational program consisting of lectures and laboratories in two existing courses, "Cell Physiology and Genomics Laboratory" and "Methods Minicourse" and an annual research symposium will promote education of the Cornell community in applications of flow cytometry. The flow cytometry funding will also sponsor a regular users meeting. Secretarial support for these courses, the program and the user meetings, including announcements, technical bulletins, and course materials, will be provided by Biomedical Sciences. Continuing institutional commitment to flow cytometry will be provided as $150,000 over the first three years and a guaranteed service contract thereafter for the life of the instrument. The instrument requested is a Dako-Cytomation basic MoFlo plus a UV-vis, vis, and HeNe laser to provide independent UV, vis/488, and red excitation.