Fluorescence activated cell sorter

描述（由申请人提供）：该提案寻求支持，以在康奈尔大学获得荧光激活细胞分选仪，该大学不再有活细胞分选仪。该仪器将支持一个用户组，目前由22名教师组成，他们拥有32项NIH研究赠款，加上几项NSF和USDA/NRI赠款。他们还拥有3个NIH（T32）培训赠款。因此，该仪器将满足这些NIH赞助的研究项目对细胞计数的需求，提高正在进行的研究的生产力，并增强培训计划，然后可以包括细胞计数。22名教师将在广泛的研究中使用细胞计数。这些包括细胞周期调节、细胞因子和激素的信号传导、对感染的细胞应答、钙动员、凋亡、白细胞分型、线粒体功能、细胞分化、异位基因表达和转录调节。流式细胞仪设施将由Andrew Yen负责。他在斯隆-凯特琳研究所当研究生时就有做流式细胞术的经验，当时第一台商业机器（BioPhysics，Mahopac，NY）是在他的导师的实验室中获得的。他仍在流式细胞仪的应用中担任实验室工作。一位经验丰富的技术人员将做日常运行，主任将做故障排除和实验设计，以及数据解释，与用户。组织结构将包括一名主任和一个指导委员会，该委员会由用户社区的6名轮流成员以及研究和研究生教育主任组成。使用费将收取和会计支持的设施将由该部。生物医学科学。一个教育计划包括两个现有课程的讲座和实验室，“细胞生理学和基因组学实验室”和“方法迷你课程”以及年度研究研讨会将促进康奈尔大学社区在流式细胞术应用方面的教育。流式细胞术资金还将赞助定期用户会议。这些课程、计划和用户会议的秘书支持，包括公告、技术公告和课程材料，将由生物医学科学提供。将在头三年为流式细胞仪提供150 000美元的持续机构承诺，并在其后的仪器寿命期间提供有保证的服务合同。所需的仪器是Dako-Cytomation basic MoFlo加上UV-vis、维斯和HeNe激光器，以提供独立的UV、维斯/488和红色激发。

DESCRIPTION (provided by applicant): This proposal seeks support to acquire a fluorescence activated cell sorter at Cornell University where there is no longer a viable cell sorter. The instrument will support a user group currently consisting of 22 faculty who have 32 NIH research grants plus several NSF and USDA/NRI grants. They also have 3 NIH (T32) training grants. The instrument will thus fulfill needs for cytometry in these NIH sponsored research programs, enhancing productivity of ongoing research and augmenting the training programs, which can then include cytometry. The 22 faculty will use cytometry in a wide range of studies. These include cell cycle regulation, signaling by cytokines and hormones, cellular response to infection, calcium mobilization, apoptosis, leukocyte typing, mitochondrial function, cell differentiation, ectopic gene expression, and transcriptional regulation. The flow cytometry facility will be directed by Andrew Yen. He has had experience doing flow cytometry since he was a graduate student at the Sloan- Kettering Institute when the first commercial machines (BioPhysics, Mahopac, NY) were acquired in the laboratory of his mentors. He is still at the bench in flow cytometric applications. An experienced technician will do day-to-day running, and the director will do trouble shooting and experimental design, as well as data interpretation, with users. The organizational structure will consist of a director and a guidance committee made of 6 rotating members of the user community and the Director of Research and Graduate Education. User fees will be charged and accounting support for the facility will be by the Dept. of Biomedical Sciences. An educational program consisting of lectures and laboratories in two existing courses, "Cell Physiology and Genomics Laboratory" and "Methods Minicourse" and an annual research symposium will promote education of the Cornell community in applications of flow cytometry. The flow cytometry funding will also sponsor a regular users meeting. Secretarial support for these courses, the program and the user meetings, including announcements, technical bulletins, and course materials, will be provided by Biomedical Sciences. Continuing institutional commitment to flow cytometry will be provided as $150,000 over the first three years and a guaranteed service contract thereafter for the life of the instrument. The instrument requested is a Dako-Cytomation basic MoFlo plus a UV-vis, vis, and HeNe laser to provide independent UV, vis/488, and red excitation.