Fluorescence activated cell sorter

描述（由申请人提供）：本提案寻求支持在康奈尔大学购买荧光激活细胞分选仪，该大学不再有可行的细胞分选仪。该仪器将支持目前由 22 名教员组成的用户组，他们拥有 32 项 NIH 研究资助以及多项 NSF 和 USDA/NRI 资助。他们还拥有 3 项 NIH (T32) 培训补助金。因此，该仪器将满足 NIH 资助的研究项目中对细胞计数的需求，提高正在进行的研究的生产力并增强培训计划，其中可以包括细胞计数。这 22 名教员将在广泛的研究中使用细胞计数术。这些包括细胞周期调节、细胞因子和激素信号传导、细胞对感染的反应、钙动员、细胞凋亡、白细胞分型、线粒体功能、细胞分化、异位基因表达和转录调节。流式细胞术设施将由 Andrew Yen 领导。自从他在斯隆凯特琳研究所攻读研究生时，他就拥有了进行流式细胞术的经验，当时他的导师的实验室购买了第一台商用机器（BioPhysics，Mahopac，NY）。他仍处于流式细胞术应用领域的替补地位。经验丰富的技术人员将负责日常运行，主管将与用户一起进行故障排除和实验设计以及数据解释。组织结构将由一名主任和一个由 6 名用户社区轮流成员组成的指导委员会以及研究和研究生教育主任组成。该设施将收取使用费，并由生物医学科学部提供会计支持。由两门现有课程“细胞生理学和基因组学实验室”和“方法迷你课程”的讲座和实验室组成的教育计划以及年度研究研讨会将促进康奈尔大学社区在流式细胞术应用方面的教育。流式细胞术资金还将赞助定期用户会议。这些课程、计划和用户会议的秘书支持，包括公告、技术公告和课程材料，将由生物医学科学提供。机构对流式细胞术的持续承诺将在前三年提供 150,000 美元，并在仪器的整个使用寿命内提供有保障的服务合同。所需的仪器是 Dako-Cytomation 基本 MoFlo 加上 UV-vis、vis 和 HeNe 激光器，以提供独立的 UV、vis/488 和红色激发。

DESCRIPTION (provided by applicant): This proposal seeks support to acquire a fluorescence activated cell sorter at Cornell University where there is no longer a viable cell sorter. The instrument will support a user group currently consisting of 22 faculty who have 32 NIH research grants plus several NSF and USDA/NRI grants. They also have 3 NIH (T32) training grants. The instrument will thus fulfill needs for cytometry in these NIH sponsored research programs, enhancing productivity of ongoing research and augmenting the training programs, which can then include cytometry. The 22 faculty will use cytometry in a wide range of studies. These include cell cycle regulation, signaling by cytokines and hormones, cellular response to infection, calcium mobilization, apoptosis, leukocyte typing, mitochondrial function, cell differentiation, ectopic gene expression, and transcriptional regulation. The flow cytometry facility will be directed by Andrew Yen. He has had experience doing flow cytometry since he was a graduate student at the Sloan- Kettering Institute when the first commercial machines (BioPhysics, Mahopac, NY) were acquired in the laboratory of his mentors. He is still at the bench in flow cytometric applications. An experienced technician will do day-to-day running, and the director will do trouble shooting and experimental design, as well as data interpretation, with users. The organizational structure will consist of a director and a guidance committee made of 6 rotating members of the user community and the Director of Research and Graduate Education. User fees will be charged and accounting support for the facility will be by the Dept. of Biomedical Sciences. An educational program consisting of lectures and laboratories in two existing courses, "Cell Physiology and Genomics Laboratory" and "Methods Minicourse" and an annual research symposium will promote education of the Cornell community in applications of flow cytometry. The flow cytometry funding will also sponsor a regular users meeting. Secretarial support for these courses, the program and the user meetings, including announcements, technical bulletins, and course materials, will be provided by Biomedical Sciences. Continuing institutional commitment to flow cytometry will be provided as $150,000 over the first three years and a guaranteed service contract thereafter for the life of the instrument. The instrument requested is a Dako-Cytomation basic MoFlo plus a UV-vis, vis, and HeNe laser to provide independent UV, vis/488, and red excitation.