Linking c-di-GMP signalling and the Gac/Rsm signal transduction pathway
Linking c-di-GMP signalling and the Gac/Rsm signal transduction pathway
批准号:
BB/L007959/1
负责人:
Alain Filloux
金额:
$51.46万
依托单位:
依托单位国家:
英国
项目类别:
Research Grant
财政年份:
2014
资助国家:
英国
项目状态:
已结题
起止时间:
2014 至 --
中文摘要
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英文摘要
Pseudomonas aeruginosa is a gram-negative bacterium that thrives in a multitude of environmental niches including soil, moist surfaces or plants. It is also an opportunistic human pathogen causing infections associated with high levels of morbidity and mortality. Epidemiological studies have shown that no significant differences in the virulence potential of clinical or environmental isolates could be observed, which suggests that the available arsenal of virulence factors might also be useful to fight eukaryotic predators that may be encountered in the environment (e.g. protozoans and metazoans). As such, P. aeruginosa is a versatile organism and its adaptive potential to fluctuating environmental cues relies on numerous and complex signalling pathways. These pathways determine whether P. aeruginosa adopts a motile/toxic or sessile/biofilm lifestyle. These lifestyles directly relate to acute or chronic infection mode.The decision making process for choosing in between lifestyles is governed by major regulatory pathways which are antagonistic but also cross-talk via positive and negative feedback loops. These involve Quorum Sensing, or the second messenger molecule c-AMP, which are both master regulators for promoting production of bacterial virulence factors such as toxins. In contrast the so-called, Gac/Rsm pathway, which involves molecular sensors for probing environmental conditions, and the second messenger molecule c-di-GMP are main players to control the formation of biofilm.A biofilm is a bacterial population attached on a surface and held together by a sticky extracellular matrix made of polysaccharide, DNA or adhesive proteins. Bacteria embedded in such matrix form a very stable population which in the environment can resist various stresses such as the current of a river flow when attached on rocks, UV irradiation, dehydration or nutrient starvation for example. For pathogens, once the bacteria establish as a biofilm in one particular tissue or organ, such as in the lungs of cystic fibrosis patients in case of P. aeruginosa, it is basically impossible to eradicate. The population became highly resistant to attack by the immune system or to antibiotic therapy. This way a chronic and persistent infection establishes and morbidity reaches a very high level.In our project we will investigate the intimate connections that may exist in between the formation/dispersion of a P. aeruginosa biofilm and the two regulatory pathways Gac/Rsm on the one hand and c-di-GMP on the other hand. We want to build on our expertise and pioneering work in the characterization of the Gac/Rsm pathway and on our recent demonstration that this route intersects with the c-di-GMP signaling pathway. We want to establish the molecular links between these two main players and understand how it may then act on the formation of the biofilm and beyond this how it exerts an antagonistic action on the production of virulence factors such as toxins. Indeed, these regulatory pathways are at the intersection of a Doctor Jekyll and Mister Hyde game, in which bacteria hesitate to balance either towards an aggressive and toxic lifestyle or towards a more silent and insidious lifestyle under the form of a biofilm.In conclusion, the regulatory network we will investigate is not unique to the human pathogen P. aeruginosa but common to most gram-negative bacteria for c-di-GMP and to most Pseudomonas species for Gac/Rsm including plant growth promoters (Pseudomonas putida and Pseudomonas fluorescens) or plant pathogens (Pseudomonas syringae). We believe that a precise molecular understanding of this circuitry will allow to develop small molecules designed for manipulating the fate of bacteria, subverting and influencing their decision making process to our own benefit. This study will have strong implications in several areas including, Ecology and Agriculture (niche/root colonization), Medical and Therapeutic approaches and Fundamental and Basic Sciences.
期刊论文(10)
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DOI:
10.1039/d2cb00205a
发表时间:
2023-01-04
期刊:
RSC CHEMICAL BIOLOGY
影响因子:
4.1
作者:
[Evans, Lindsay, Kotar, Anita, Valentini, Martina, Filloux, Alain, Jamshidi, Shirin, Plavec, Janez, Rahman, Khondaker Miraz, Vilar, Ramon]
通讯作者:
Vilar, Ramon
A broad range quorum sensing inhibitor working through sRNA inhibition.
通过SRNA抑制作用的范围群体传感抑制剂。
DOI:
10.1038/s41598-017-09886-8
发表时间:
2017-08-29
期刊:
Scientific reports
影响因子:
4.6
作者:
[Jakobsen TH, Warming AN, Vejborg RM, Moscoso JA, Stegger M, Lorenzen F, Rybtke M, Andersen JB, Petersen R, Andersen PS, Nielsen TE, Tolker-Nielsen T, Filloux A, Ingmer H, Givskov M]
通讯作者:
Givskov M
DOI:
10.1128/jb.01850-14
发表时间:
2014-12
期刊:
Journal of bacteriology
影响因子:
3.2
作者:
[Moscoso JA, Jaeger T, Valentini M, Hui K, Jenal U, Filloux A]
通讯作者:
Filloux A
DOI:
10.1038/nmicrobiol.2017.27
发表时间:
2017-03-06
期刊:
Nature microbiology
影响因子:
28.3
作者:
[McCarthy RR, Mazon-Moya MJ, Moscoso JA, Hao Y, Lam JS, Bordi C, Mostowy S, Filloux A]
通讯作者:
Filloux A
DOI:
10.3389/fmicb.2015.00630
发表时间:
2015
期刊:
Frontiers in microbiology
影响因子:
5.2
作者:
[Bouffartigues E, Moscoso JA, Duchesne R, Rosay T, Fito-Boncompte L, Gicquel G, Maillot O, Bénard M, Bazire A, Brenner-Weiss G, Lesouhaitier O, Lerouge P, Dufour A, Orange N, Feuilloley MG, Overhage J, Filloux A, Chevalier S]
通讯作者:
Chevalier S
The T6SS as a search engine for naturally validated antibacterial targets
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批准号:MR/S02316X/1
-
项目类别:Research Grant
-
资助金额:$67.78万
-
财政年份:2019
-
负责人:Alain Filloux
-
依托单位:
A bacterial c-di-GMP responsive enzyme modulates LPS structure and triggers immune evasion
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批准号:BB/R00174X/1
-
项目类别:Research Grant
-
资助金额:$61.98万
-
财政年份:2018
-
负责人:Alain Filloux
-
依托单位:
Type VI secretion in Pseudomonas species: bacterial competition and biocontrol
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批准号:BB/N002539/1
-
项目类别:Research Grant
-
资助金额:$55.44万
-
财政年份:2016
-
负责人:Alain Filloux
-
依托单位:
The T6SS toxins are powerful weapons for Pseudomonas' antibacterial strategy
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批准号:MR/N023250/1
-
项目类别:Research Grant
-
资助金额:$58.04万
-
财政年份:2016
-
负责人:Alain Filloux
-
依托单位:
Bacterial competition in planta: The Type 6 Secretion System (T6SS) paradigm
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批准号:BB/M02735X/1
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项目类别:Research Grant
-
资助金额:$3.16万
-
财政年份:2015
-
负责人:Alain Filloux
-
依托单位:
Structure and function of the Pseudomonas aeruginosa type VI secretion system: On the bacteriophage trail
-
批准号:MR/K001930/1
-
项目类别:Research Grant
-
资助金额:$180.54万
-
财政年份:2013
-
负责人:Alain Filloux
-
依托单位:
The P-Usher: A mix and match secretion machine for the assembly of bacterial cell surface appendages.
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批准号:BB/I019871/1
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项目类别:Research Grant
-
资助金额:$44.45万
-
财政年份:2011
-
负责人:Alain Filloux
-
依托单位:
Pseudomonas aeruginosa infection: analysis of antigenic proteins of the virulence-associated type VI secretion system
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批准号:G0800171/1
-
项目类别:Research Grant
-
资助金额:$67.21万
-
财政年份:2008
-
负责人:Alain Filloux
-
依托单位:
Signalling pathway controlling cupD fimbrial genes expression and role in Pseudomonas aeruginosa pathogenesis
-
批准号:BB/F019645/1
-
项目类别:Research Grant
-
资助金额:$44.43万
-
财政年份:2008
-
负责人:Alain Filloux
-
依托单位:
国内基金
海外基金
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