Pseudomonas aeruginosa infection: analysis of antigenic proteins of the virulence-associated type VI secretion system

铜绿假单胞菌感染：毒力相关 VI 型分泌系统抗原蛋白分析

• 批准号: G0800171/1
• 负责人: Alain Filloux
• 金额: $ 67.21万
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2008
• 资助国家: 英国
• 起止时间: 2008 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=G0800171%2F1
• 关键词: Pseudomonas; aeruginosa; infection; analysis; antigenic

Interaction between microbes and human hosts can range from a benign, even commensal collaboration to a competition that may turn fatal resulting in the death of the host, the bacteria or both. The outcome of the interaction is balanced by a communication/signalling game in between the host and the pathogen. Among the arsenal of communication tools used by bacteria to influence host response are secretion systems (SS), which deliver toxins to the host. The SS and the transported toxins are key players in bacterial pathogenesis. Recent studies have seen the emergence of a novel type of SS, named T6SS. Bacteria harbouring T6SS all have a life style where they live in intimate contact with a host.Pseudomonas aeruginosa is an opportunistic pathogen. It causes urinary tract infections, respiratory system infections, dermatitis, soft tissue infections, bacteremia, bone and joint infections, gastrointestinal infections and a variety of systemic infections, particularly in immuno-suppressed patients. P. aeruginosa infection is a serious problem in patients hospitalized with cancer, cystic fibrosis, and burns. The case fatality rate in these patients is 50 percent. P. aeruginosa is the third most commonly-isolated nosocomial pathogen accounting for 10% of all hospital-acquired infections.P. aeruginosa possesses 3 T6SS. The goal of our study is to understand the molecular mechanisms, which are involved in the T6SS functioning, knowledge applicable to other T6SS-containing bacterial pathogens. We will further use this knowledge to develop antimicrobials, which will help fighting human morbidity and mortality due to persistent bacterial infections.

微生物和人类宿主之间的相互作用可以是良性的，甚至是竞争性的合作，也可以是致命的竞争，导致宿主、细菌或两者的死亡。相互作用的结果通过宿主和病原体之间的通信/信号博弈来平衡。细菌用来影响宿主反应的通讯工具包括分泌系统（SS），它将毒素传递给宿主。SS和转运的毒素是细菌致病的关键参与者。最近的研究发现了一种新型的SS，称为T6 SS。携带T6 SS的细菌都有一种与宿主密切接触的生活方式。铜绿假单胞菌是一种条件致病菌。它引起尿路感染、呼吸系统感染、皮炎、软组织感染、菌血症、骨和关节感染、胃肠道感染和各种全身感染，特别是在免疫抑制患者中。铜绿假单胞菌感染是癌症、囊性纤维化和烧伤住院患者的严重问题。这些患者的病死率为50%。铜绿假单胞菌是第三常见的医院内病原体，占所有医院获得性感染的10%。铜绿假单胞菌具有3个T6 SS。我们研究的目的是了解参与T6 SS功能的分子机制，这些知识适用于其他含T6 SS的细菌病原体。我们将进一步利用这些知识来开发抗菌剂，这将有助于对抗由于持续性细菌感染引起的人类发病率和死亡率。