The T6SS toxins are powerful weapons for Pseudomonas' antibacterial strategy

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Bacteria have evolved plenty of strategies to thrive within harsh environments, to colonize hostile hosts and to compete with other microorganisms in a fierce race for goods and survival. Bacterial pathogens that infect humans can use various strategies. They develop acute infections, which turn fatal in a short period of time, but also establish chronic infections and persist within the host over a lifetime. That is the case for lung infections in cystic fibrosis (CF) patients. At early stages of infection, diverse bacteria are present, e.g. Staphylococcus, Burkholderia or Pseudomonas aeruginosa. At later stages in the patient life the sole microorganism left is P. aeruginosa, which is chronically established and will lead to patient death. The ability of P. aeruginosa to establish chronic infection is not exclusive to CF or pulmonary diseases but also results from contamination of medical devices used in clinical set up and on which P. aeruginosa establish a resilient biofilm barely sensitive to antibiotic treatments. From there it disseminates in the human body and causes high level of morbidity and mortality.There are multiple reasons for which P. aeruginosa is such a successful colonizer and pathogenic organism. However, one recent discovery pointed to its ability to use a molecular weapon, which facilitates the elimination of bacterial competitors such as in the CF lung colonization. This weapon is the type VI secretion system (T6SS) and has the design of a puncturing device perforating the envelope of target organisms in order to allow injection of lethal toxins. To protect itself from T6SS toxins the bacterium produces an antidote, or immunity, thus preventing self-killing and only aiming at non-self organisms lacking these immunities.The repertoire of T6SS toxins is currently undervalued and it will be invaluable to decipher the complete T6SS toxin armoury for several reasons. In the first place what makes the T6SS a potent weapon is not the gun but the toxin bullets it fires. How many and how distinct these toxins are will define how potent and efficient in colonization the bacterium is. We will use a completely randomized approach (TraDIS) to identify all T6SS toxin/immunity pairs. Using this knowledge we will be in a position to determine the T6SS profile of any clinical isolates and thus gain knowledge and understanding on why specific isolates may be worse than another. The cartography of the T6SS landscape could be an indicator of the context and severity of the infection. Secondly, the identification of toxins of unknown function could result in the development of new drugs that we desperately need considering the exhaustion of the antibiotic pipeline. In other words a T6SS toxin that kill bacteria by aiming at a yet unexplored target will provide pharmaceutical companies with a novel venue to explore and the possibility for drug design. Since P. aeruginosa possesses three distinct T6SSs, each potentially firing dozens of distinct toxins, the potential for new discoveries is huge and exciting.In summary, not only this project aims at unraveling the repertoire of T6SS toxin/immunity pairs available to P. aeruginosa, but it also aims (i) at understanding whether these toxins are fired at once and simultaneously by all three T6SS machines, (ii) at visualizing how the T6SS docks onto prey cells and how toxins travels through it, (iii) at visualising the transport of the toxins using super resolution fluorescence microscopy, (iv) at applying this knowledge to clinical isolates from CF patients in monitoring a correlation between high T6SS potency and high capability of host colonisation and persistence. Basic knowledge about the T6SS mechanism is crucial since one can foresee the possibility to elaborate live innocuous organisms for human kind, which could be equipped with a specific T6SS weaponry able to eliminate bacterial pathogens, and this will only be possible by having such in depth understanding.