CELL CYCLE SPECIFIC CONTROL OF CELLULAR DIFFERENTIATION

细胞分化的细胞周期特异性控制

• 批准号: 3171359
• 负责人: ANDREW YEN
• 金额: $ 10.82万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-08-01 至 1991-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3171359
• 关键词: blood /lymphatic neoplasm; blood tests; cell differentiation; cell growth regulation; cell population study; cellular oncology; flow cytometry; human subject; leukemia; mathematical model; mitogens; myeloid stem cell; neoplastic growth; nucleic acid hybridization; nucleic acid probes; oncogenes; synchronous cell division

These studies investigate the regulation of cellular differentiation. The specific primary objectives are to determine: (1)\the amount of anteceding proliferation needed for differentiation; (2)\the cell cycle specificity of cellular processes regulating differentiation; and (3)\whether a central or diverse cellular regulatory process(es) effects different courses of differentiation in a precursor cell. The cell system to be used is the HL-60 human promyelocytic leukemia cell line. Subject to different inducers, HL-60 differentiates along either the myeloid or monocytic pathways. The cell-cycle kinetics and the kinetics of differentiation effected by different inducers will be determined using multiparameter flow cytometric analysis. The cell-cycle specificity of regulatory processes committing this precursor cell to myeloid or monocytic pathways of differentiation will be determined using synchronized cells. The primary scientific questions addressed are: (1)\how much proliferative activity precedes cellular differentiation; (2)\what cellular processes in the proliferative cell cycle are implicated with a regulatory role for effecting differentiation; and (3)\does the same or different cellular-regulatory process(es) govern differentiation along different pathways? These studies directed at understanding the cellular-regulatory mechanism of differentiation are of particular relevance to the pathology of hematological neoplasms. The cellular defect seminal to leukemogenesis is attributable to a pathological differentiative process. The studies should increase the understanding of that pathology and hopefully promote insight into new means of more effective clinical management of such diseases. Of relevance to this is the utilization of these studies of agents which modulate cellular differentiation. Understanding the cellular response to such agents may lead to treatment protocols utilizing these or related compounds. (N)

这些研究调查细胞分化的调节。 的 具体的主要目标是确定：（1）前序量 分化所需的增殖;（2）细胞周期特异性 调节分化的细胞过程;以及（3）\是否是中央或 不同的细胞调节过程影响不同的过程， 前体细胞的分化。 要使用的细胞系统是 HL-60人早幼粒细胞白血病细胞系。 受到不同 诱导剂，HL-60沿着髓系或单核细胞分化 途径。 细胞周期动力学和分化动力学 将使用多参数流来确定不同诱导剂的影响 细胞计数分析。 调节过程的细胞周期特异性 使这种前体细胞进入骨髓或单核细胞途径， 将使用同步化的细胞来确定分化。 主 提出的科学问题是：（1）增殖活性有多大 （2）在细胞分化之前;（3）在细胞分化过程中， 增殖细胞周期与调节作用， （3）是否相同或不同 细胞调节过程控制着沿着不同方向的分化 路径？ 这些研究旨在了解细胞调节 分化机制与病理学特别相关 血液肿瘤 精液细胞缺陷与白血病发生 是由病理分化过程引起的 研究 应该可以增加对这种病理学的理解， 深入了解这种更有效的临床管理的新方法， 疾病 与此相关的是利用这些研究， 调节细胞分化的试剂。 了解细胞 对这些药剂的反应可导致利用这些或 相关化合物。 （N）个