TRANSFORMING SEQUENCES OF YABA VIRUS DNA

这一建议涉及的是 转化DNA序列的Yaba猴肿瘤痘病毒。 存在的因果关系研究 病毒DNA序列，它们的表达， 将使用回复突变体制备细胞的状态，以测试 病毒DNA序列的存在和使用来自 转化细胞转化其他细胞。 病毒鉴定 负责转化的DNA序列将 最初通过筛选独立转化的细胞系， 共同的DNA片段，并通过用病毒 从转化细胞中分离出的DNA片段， 串行转换将导致失去非线性，非本质的 转化细胞中的病毒DNA序列。 的物理状态 转化细胞核中的Yaba病毒特异性DNA将 以游离存在于细胞核中或整合到 细胞DNA或者两者都有 病毒DNA序列的精细定位 将通过亚克隆克隆研究转化细胞中发现的 在转化细胞中发现的DNA片段，并使用北方 印迹杂交和体外翻译鉴定 使者 合成的蛋白质将与 转化细胞 病毒DNA在细胞转染中的作用 将通过Balb/c 3 T3的肿瘤转化来研究细胞 Yaba病毒DNA片段将用于鉴定Yaba DNA 转化细胞基因组中的序列。 的可能性 亚巴病毒的转化DNA序列存在于 其他痘病毒将被研究。 的遗传表达 在转化细胞中发现的亚巴病毒DNA序列将被 研究了 将检测用Yaba病毒转化的细胞， 它们在仓鼠、老鼠和猴子身上形成肿瘤的能力。 Yaba转化DNA序列的表征 病毒应该阐明更多的信息，这将导致 确定Yaba的转化机制 病毒 这一信息特别令人感兴趣，因为Yaba 病毒在其自然宿主猴子体内是致癌的， 人的肿瘤

This proposal is concerned with the characterization of the transforming DNA sequences of Yaba monkey tumor pox virus. Study of the cause and effect relationship between the presence of viral DNA sequences, their expression, and the transformed state of the cells will be made using revertants to test for the presence of viral DNA sequences and using DNA from transformed cells to transform other cells. Identification of viral DNA sequences responsible for transformation will be made initially by screening independently transformed cell lines for common DNA segments and by serial transformation with viral DNA segments isolated from transformed cells on the theory that serial transformation will lead to loss of unlined, nonessential viral DNA sequences in transformed cells. The physical state of Yaba virus specific DNA in the nucleus of transformed cells will be characterized as existing free in the nucleus or integrated into the cellular DNA or both. Fine mapping of viral DNA sequences found in transformed cells will be studied by subcloning cloned DNA fragments found in transformed cells and using Northern blot hybridization and in vitro translation of identified messengers. Proteins synthesized will be compared to those in transformed cells. The role of viral DNA in the transfection of cells will be studied by neoplastic transformation of Balb/c 3T3 fragments of Yaba virus DNA will be used to identify Yaba DNA sequences in the genome of transformed cells. The possibility that the transforming DNA sequences of Yaba virus are found in other pox viruses will be investigated. Genetic expression of Yaba virus DNA sequences found in transformed cells will be studied. Cells transformed with Yaba virus will be tested for their ability to form tumors in hamsters, mice and monkeys. Characterization of the transforming DNA sequences of Yaba virus should elucidate additional information that will lead to the identification of the mechanism(s) of transformation by Yaba virus. This information is of particular interest because Yaba virus is oncogenic in monkeys, its natural host, and it produces tumors in man.