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TRANSFORMING SEQUENCES OF YABA VIRUS DNA

雅巴病毒 DNA 序列的转化

基本信息

批准号：
3176861
负责人：
HASSAN ROUHANDEH
金额：
$ 10.83万
依托单位：
SOUTHERN ILLINOIS UNIVERSITY CARBONDALE
依托单位国家：
美国
项目类别：
财政年份：
1987
资助国家：
美国
起止时间：
1987-01-01 至 1990-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3176861
关键词：
Pongidae gene expression genetic mapping genetic translation genome hamsters kidney cell laboratory mouse molecular cloning nucleic acid sequence oncogenic virus tissue /cell culture transforming virus viral carcinogenesis virus DNA virus classification virus genetics virus replication

项目摘要

This proposal is concerned with the characterization of the transforming DNA sequences of Yaba monkey tumor pox virus. Study of the cause and effect relationship between the presence of viral DNA sequences, their expression, and the transformed state of the cells will be made using revertants to test for the presence of viral DNA sequences and using DNA from transformed cells to transform other cells. Identification of viral DNA sequences responsible for transformation will be made initially by screening independently transformed cell lines for common DNA segments and by serial transformation with viral DNA segments isolated from transformed cells on the theory that serial transformation will lead to loss of unlined, nonessential viral DNA sequences in transformed cells. The physical state of Yaba virus specific DNA in the nucleus of transformed cells will be characterized as existing free in the nucleus or integrated into the cellular DNA or both. Fine mapping of viral DNA sequences found in transformed cells will be studied by subcloning cloned DNA fragments found in transformed cells and using Northern blot hybridization and in vitro translation of identified messengers. Proteins synthesized will be compared to those in transformed cells. The role of viral DNA in the transfection of cells will be studied by neoplastic transformation of Balb/c 3T3 fragments of Yaba virus DNA will be used to identify Yaba DNA sequences in the genome of transformed cells. The possibility that the transforming DNA sequences of Yaba virus are found in other pox viruses will be investigated. Genetic expression of Yaba virus DNA sequences found in transformed cells will be studied. Cells transformed with Yaba virus will be tested for their ability to form tumors in hamsters, mice and monkeys. Characterization of the transforming DNA sequences of Yaba virus should elucidate additional information that will lead to the identification of the mechanism(s) of transformation by Yaba virus. This information is of particular interest because Yaba virus is oncogenic in monkeys, its natural host, and it produces tumors in man.

该提案涉及的特征转化雅巴猴瘤痘病毒的 DNA 序列。研究存在之间的因果关系病毒DNA序列、它们的表达以及转化的将使用回复体来测试细胞的状态病毒 DNA 序列的存在并使用来自转化细胞来转化其他细胞。病毒鉴定将制作负责转化的DNA序列最初通过筛选独立转化的细胞系常见的 DNA 片段以及通过病毒的连续转化从转化细胞中分离出的 DNA 片段的理论是串行转换会导致失去内衬的、非必要的转化细胞中的病毒DNA序列。身体状态转化细胞核中的雅巴病毒特异性 DNA 将被表征为游离存在于细胞核中或整合到细胞 DNA 或两者。病毒 DNA 序列的精细绘制在转化细胞中发现的克隆将通过亚克隆进行研究在转化细胞中发现的 DNA 片段并使用 Northern 印迹杂交和鉴定的体外翻译使者。合成的蛋白质将与中的蛋白质进行比较转化的细胞。病毒DNA在转染中的作用将通过 Balb/c 3T3 的肿瘤转化来研究细胞雅巴病毒DNA片段将用于鉴定雅巴DNA 转化细胞基因组中的序列。可能性雅巴病毒的转化DNA序列被发现于其他痘病毒也将受到调查。基因表达在转化细胞中发现的雅巴病毒 DNA 序列将被研究过。将测试用雅巴病毒转化的细胞它们在仓鼠、小鼠和猴子中形成肿瘤的能力。 Yaba 转化 DNA 序列的表征病毒应该阐明额外的信息，从而导致 Yaba 识别转化机制病毒。此信息特别令人感兴趣，因为 Yaba 病毒在其自然宿主猴子体内具有致癌性，并产生人体肿瘤。