NATURALLY OCCURRING ANTICANCER COMPOUNDS

天然存在的抗癌化合物

• 批准号: 3173425
• 负责人: JOHN H CARDELLINA
• 金额: $ 8.08万
• 依托单位: MONTANA STATE UNIVERSITY - BOZEMAN
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-07-01 至 1987-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3173425
• 关键词: DNA repair; X ray crystallography; chemical structure function; drug screening /evaluation; infrared spectrometry; mass spectrometry; medicinal plants; nuclear magnetic resonance spectroscopy; plant extracts; saltwater environment; ultraviolet spectrometry

The long term objective of this project is to develop new agents for cancer chemotherapy from natural sources. To attain this goal, cytotoxicity or antitumor/antineoplastic activity will be pinpointed in terrestrial plants and marine fauna and flora; the compounds eliciting the observed activity will then be isolated, identified and submitted for full pharmacological evaluation. Screening and bioassay work will follow NCI protocols and will be provided by the National Cancer Institute and the Lilly Research Laboratories. In addition, an assay for toxicity to bacteria deficient in DNA repair capacity will be utilized whenever feasible to save time and material. Separation work will be accomplished by application of every available chromatographic technique; modifications and new adaptations in this area have been and will continue to be developed by the principal investigator. Elucidation of structures will be acomplished by utilization of state-of-the-art spectroscopic techniques, particularly NMR, mass spectrometry and X-ray diffraction, supported by more traditional methods, including IR and UV spectroscopy and chemical degradations and transformations. While chemical investigations proceed on those species already targeted by screening, new species will be continuously collected and screened, in order to maintain a constant supply of extracts with potential anticancer activity.

该项目的长期目标是开发新的抗癌药物 天然来源的化疗。 为了实现这一目标，细胞毒性或 抗肿瘤/抗肿瘤活性将在陆生植物中被确定 以及海洋动物和植物群;引起所观察到的活性的化合物 然后将被分离、鉴定并提交进行全面的药理学研究。 评价 筛选和生物测定工作将遵循NCI方案，并将提供 由美国国家癌症研究所和礼来研究实验室进行。 在 此外，对DNA修复缺陷的细菌的毒性测定 在可行的情况下，将尽可能利用能力，以节省时间和材料。 分离工作将通过应用所有可用的 色谱技术;在这一领域的改进和新的调整 已经并将继续由主要研究者开发。 结构的阐明将通过利用 最先进的光谱技术，特别是核磁共振、质量 光谱和X射线衍射，由更传统的方法支持， 包括红外和紫外光谱和化学降解， 转变 虽然化学调查继续对这些物种已经针对 筛选，新物种将不断收集和筛选， 为了保持具有潜在抗癌活性的提取物的恒定供应， 活动