HUMAN AND MURINE HYBRIDOMA ANTIBODIES IN ACUTE LEUKEMIAS

急性白血病中的人和鼠杂交瘤抗体

• 批准号: 3172843
• 负责人: LENNART OLSSON
• 金额: $ 10.53万
• 依托单位: UNIVERSITY OF COPENHAGEN
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-09-15 至 1986-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3172843
• 关键词: B lymphocyte; antibody specificity; bone marrow transplantation; enzyme linked immunosorbent assay; flow cytometry; high performance liquid chromatography; human tissue; hybridomas; methylation; monoclonal antibody; myelogenous leukemia; myeloid stem cell; neoplasm /cancer genetics; neoplasm /cancer immunodiagnosis; neoplasm /cancer immunotherapy; neoplastic transformation; radiotracer; tissue /cell culture; tumor antigens

The goal of this research is the characterization of human myeloid leukemia cells using murine and human monoclonal antibody techniques to provide reagents that will be useful in the diagnosis and treatment of human myeloid leukemia. Monoclonal antibodies will be produced against cell surface antigens on normal and malignant myeloid cells with emphasis on myeloid precursor cells and their differentiation products. This technology also includes recently developed procedures to expand normal antibody-producing B-lymphocyte cultures in large quantities by demethylation of cytosin in certain nucleotide sequences. The antigenic determinants for such antibodies will be biochemically characterized. Also included will be studies on the modulation potential of such antigens. (2)

本研究的目的是人类髓性白血病的特征

项目成果

Non-specific binding of protein-stabilized gold sols as a source of error in immunocytochemistry.

蛋白质稳定金溶胶的非特异性结合是免疫细胞化学中的错误来源。

• 发表时间: 1986
• 期刊: European journal of cell biology
• 影响因子: 6.6
• 作者: Behnke,O;Ammitzboll,T;Jessen,H;Klokker,M;Nilausen,K;Tranum-Jensen,J;Olsson,L
• 通讯作者: Olsson,L

Human monoclonal antibodies: methods of production and some aspects of their application in oncology.

人单克隆抗体：生产方法及其在肿瘤学中应用的某些方面。

• DOI: 10.1007/bf02934529
• 发表时间: 1984
• 期刊: Medical oncology and tumor pharmacotherapy
• 作者: Olsson,L

Emergence of a retrovirus in a cloned cell line established from a lesion of Hodgkin's disease.

从霍奇金病病变建立的克隆细胞系中出现逆转录病毒。

• DOI: 10.1002/hon.2900060304
• 发表时间: 1988
• 期刊: Hematological oncology
• 影响因子: 3.3
• 作者: Olsson,L;Behnke,O
• 通讯作者: Behnke,O

Generation of stable cellular phenotypes in a human malignant cell line conditioned by alterations in the cellular microenvironment.

在细胞微环境改变的条件下，在人类恶性细胞系中产生稳定的细胞表型。

• 发表时间: 1986
• 期刊: Cancer research
• 影响因子: 11.2
• 作者: Honsik,CJ;Diamant,M;Olsson,L
• 通讯作者: Olsson,L

Treatment of human cell lines with 5-azacytidine may result in profound alterations in clonogenicity and growth rate.

• DOI: 10.1083/jcb.100.2.508
• 发表时间: 1985-02
• 期刊: The Journal of cell biology
• 作者: Olsson L;Due C;Diamant M
• 通讯作者: Diamant M