HUMAN AND MURINE HYBRIDOMA ANTIBODIES IN ACUTE LEUKEMIAS

本研究的目的是描述人类髓系白血病的特征 使用鼠和人单克隆抗体技术， 将用于诊断和治疗人类疾病的试剂 粒细胞白血病 单克隆抗体将针对细胞表面抗原产生， 正常和恶性骨髓细胞，重点是骨髓前体细胞 和他们的差异化产品。 这项技术还包括最近 开发程序来扩增正常的产生抗体的B淋巴细胞 通过某些细胞中的胞嘧啶的去甲基化来大量培养 核苷酸序列。这些抗体的抗原决定簇将 具有生物化学特征。 此外，还将研究 这些抗原的调节潜力。 （二）

The goal of this research is the characterization of human myeloid leukemia cells using murine and human monoclonal antibody techniques to provide reagents that will be useful in the diagnosis and treatment of human myeloid leukemia. Monoclonal antibodies will be produced against cell surface antigens on normal and malignant myeloid cells with emphasis on myeloid precursor cells and their differentiation products. This technology also includes recently developed procedures to expand normal antibody-producing B-lymphocyte cultures in large quantities by demethylation of cytosin in certain nucleotide sequences. The antigenic determinants for such antibodies will be biochemically characterized. Also included will be studies on the modulation potential of such antigens. (2)