ANALYSIS OF HUMAN NK FUNCTION

我们的研究产生了单克隆抗体， 克隆的人类NK细胞系的溶解活性。 这些抗体， 来源于与用NK克隆免疫的小鼠的脾细胞的融合， 在杀伤细胞水平上影响溶解。 其中两种抗体 检测I类MHC基因产物，另外两个检测II类MHC基因产物 产品;他们显然是独一无二的，其他反类I或II类 抗体对NK功能没有这种影响。 这表明MHC 基因产物可能以某种中心方式参与NK功能， 作为原始的受体结构。 它进一步提出了具体的 为什么这四种抗体，或者它们检测到的表位， 独特. 这些发现与细胞表面 分子如MHC糖蛋白可以在免疫系统中发挥其功能作用。 不同细胞的生理学通过不同的结构域， 分子。 我们的计划是利用 良好表征的人NK克隆和一组抗I类、抗I类、抗I类、抗I类和抗I类抗体。 II，以及检测不同决定簇的抗LFA-1单克隆抗体 对NK细胞功能有不同的影响。 （中文）

Our research derived monoclonal antibodies which either enhance or inhibit the lytic activity of a cloned iine of human NK cells. These antibodies, derived from fusions with spleen cells of mice immunized with the NK clone, affect lysis at the level of the killer cell. Two of these antibodies detect class I MHC gene products and two others detect class II MHC gene products; they are apparently unique in that other anticlass I or class II antibodies have no such effects on NK function. This suggests that MHC gene products are involved in some central way in NK function, perhaps serving as primitive receptor structures. It further raises the specific question why these four antibodies, or the epitopes they detect, are so unique. These findings are consistent with a model that a cell surface molecule such as an MHC glycoprotein may exert its functional role in the physiology of different cells via different structural domains of that molecule. It is our plan to examine this possibility using well-characterized human NK clones and a panel of anticlass I, anticlass II, and anti-LFA-1 monoclonal antibodies that detect different determinants on these molecules and which have different effects on NK function. (CS)