RADIOBIOLOGICAL STUDIES OF HUMAN HYBRID CELL LINES

本研究的目的是比较和对比 非致瘤性和致瘤性形式的放射生物学特性 另外，良好表征的人杂交细胞系（HeLa x人皮肤 成纤维细胞）。 自非致瘤性向致瘤性转化以来 表单与某些定义明确的更改（例如，特定 染色体）这些细胞系提供了一个独特的系统， 辐射敏感性的遗传控制。 此外，放射生物学研究 可能表明伴随恶性肿瘤的细胞特性的其他变化 转化（例如DNA修复能力降低）。 此外，我们 初步研究表明，这些人类杂交细胞 辐射诱导的肿瘤细胞转化的研究潜力。 因此，本建议的另一个目的是制定一种方法， 用于辐射诱导的致癌细胞的定量研究 使用杂交人类细胞进行转化。 为此提出的方法 涉及使用单克隆抗体检测恶性转化细胞， 肿瘤特异性膜蛋白抗体和荧光激活的 细胞分选

It is the aim of the proposed research to compare and contrast the radiobiological properties of the non-tumorigenic and tumorigenic forms of otherwise well characterized human hybrid cell lines (HeLa x human skin fibroblasts). Since the transformation from non-tumorigenic to tumorigenic forms is associated with certain well-defined changes (e.g. loss of specfic chromosomes) these cell lines offer a unique system with which to study the genetic control of radiosensitivity. In addition radiobiological studies may indicate oher changes in cellular properties which accompany malignant transformation (e.g. reduction in DNA repair capability). Furthermore our preliminary studies indicate that these human hybrid cells significant potential for the study of radiaton-induced ongocenic cell transformation. It is therefore an additional aim of this proposal to develop a methodology for the quantitative study of radiation-induced oncogenic cell transformation using hybrid human cells. A proposed methodology for this involves detection of malignantly transformed cells using an monoclonal antibody for a tumor-specific membrane protein and flourescence activated cell sorting.