PHOTOREACTIVATION OF X-RAY INDUCED DAMAGE IN E. COLI

X 射线引起的大肠杆菌损伤的光激活

• 批准号: 3167690
• 负责人: JOHN L REDPATH
• 金额: $ 3.47万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-08-01 至 1987-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3167690
• 关键词: DNA repair; Escherichia coli; X ray; affinity chromatography; endonuclease; ionizing radiation; light adverse effect; nonvisual photosensitivity; photoactivation; pyrimidine dimers; radiation genetics; radiation protection; radiobiology; radioimmunoassay; thymine; ultraviolet radiation

We have shown that the induction of photoreactivatable damage in E. coli AB2480 by high-energy X-ray is due to the associated Cerenkov emission. By use of various experimental techniques we have been able to maximize the yield of photoreactivatable damage as measured by cell survival with and without photoreactivation. We now propose to measure the yield of thymine dimers in DNA of X-irradiated E. coli AB2480 under similar experimental conditions to our cell survival studies. If thymine dimers are the sole photoreactivatable lesions, then survival data and thymine dimer yield measurements should correlate.

我们已经证明，光反应性损伤的诱导在E。大肠杆菌AB2480 被高能X射线辐射的原因是相关的切伦科夫发射。 通过使用 各种实验技术，我们已经能够最大限度地提高产量， 通过细胞存活率测量的光活化损伤， 光复活 我们现在建议测量DNA中胸腺嘧啶二聚体的产率 X射线照射E. coliAB 2480中进行了类似的实验条件下，我们的细胞 生存研究。 如果胸腺嘧啶二聚体是唯一的光反应性损伤， 那么存活数据和胸腺嘧啶二聚体产量测量应该相关。

项目成果

Cerenkov light and the production of photoreactivatable damage in X-irradiated E. coli.

切伦科夫光和 X 辐照大肠杆菌中光再激活损伤的产生。

• DOI: 10.1080/09553008114550681
• 发表时间: 1981
• 期刊: International journal of radiation biology and related studies in physics, chemistry, and medicine
• 作者: Redpath,JL;Zabilansky,E;Morgan,T;Ward,JF
• 通讯作者: Ward,JF

UV-type damage associated with ionizing radiation: a review.

与电离辐射相关的紫外线型损伤：综述。

• DOI: 10.1080/09553008614550581
• 发表时间: 1986
• 期刊: International journal of radiation biology and related studies in physics, chemistry, and medicine
• 作者: Redpath,JL

Further studies on Cerenkov-induced photoreactivatable damage in E. coli.

关于切伦科夫诱导的大肠杆菌光再激活损伤的进一步研究。

• 发表时间: 1982
• 期刊: Radiation research
• 影响因子: 3.4
• 作者: Morgan,TL;Redpath,JL;Ward,JF
• 通讯作者: Ward,JF

Mutation induction in Escherichia coli WP2 uvrA by Cerenkov emission associated with 137Cs gamma irradiation.

通过与 137Cs 伽玛辐射相关的切伦科夫发射诱导大肠杆菌 WP2 uvrA 突变。

• 发表时间: 1986
• 期刊: Radiation research
• 影响因子: 3.4
• 作者: Redpath,JL;Thaden,JJ;Fong,RL
• 通讯作者: Fong,RL

Induction of lethal damage in E. coli by Cerenkov emission associated with high-energy X-rays: the effect of bromouracil substitution.

与高能 X 射线相关的切伦科夫发射对大肠杆菌的致死损伤的诱导：溴尿嘧啶替代的效果。

• DOI: 10.1080/09553008414550311
• 发表时间: 1984
• 期刊: International journal of radiation biology and related studies in physics, chemistry, and medicine
• 作者: Morgan,TL;Redpath,JL;Ward,JF
• 通讯作者: Ward,JF