RADIOBIOLOGICAL STUDIES OF HUMAN HYBRID CELL LINES

人类杂交细胞系的放射生物学研究

• 批准号: 3178133
• 负责人: JOHN L REDPATH
• 金额: $ 14.31万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-03-01 至 1989-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3178133
• 关键词: DNA repair; affinity chromatography; cell sorting; cellular oncology; cytogenetics; hybrid cells; ionizing radiation; monoclonal antibody; radiation carcinogenesis; radiation dosage; radiation sensitivity; relative biological effectiveness

It is the aim of the proposed research to compare and contrast the radiobiological properties of the non-tumorigenic and tumorigenic forms of otherwise well characterized human hybrid cell lines (HeLa x human skin fibroblasts). Since the transformation from non-tumorigenic to tumorigenic forms is associated with certain well-defined changes (e.g. loss of specfic chromosomes) these cell lines offer a unique system with which to study the genetic control of radiosensitivity. In addition radiobiological studies may indicate oher changes in cellular properties which accompany malignant transformation (e.g. reduction in DNA repair capability). Furthermore our preliminary studies indicate that these human hybrid cells significant potential for the study of radiaton-induced ongocenic cell transformation. It is therefore an additional aim of this proposal to develop a methodology for the quantitative study of radiation-induced oncogenic cell transformation using hybrid human cells. A proposed methodology for this involves detection of malignantly transformed cells using an monoclonal antibody for a tumor-specific membrane protein and flourescence activated cell sorting.

这项拟议研究的目的是比较和对比 非致瘤型和致瘤型肺癌的放射生物学特性 其他方面很好地描述了人类杂交细胞系(HeLa x人皮肤 成纤维细胞)。自非致瘤性向致瘤性转化以来 表单与某些定义明确的更改相关联(例如，丢失特定 染色体)这些细胞系提供了一种独特的系统来研究 辐射敏感性的遗传控制。此外，放射生物学研究 可能表明伴随着恶性疾病的细胞特性的过度改变 转化(例如，DNA修复能力降低)。此外，我们的 初步研究表明，这些人类杂交细胞具有重要意义 辐射诱导成瘤细胞转化研究的潜力。 因此，这项提议的另一个目的是制定一种方法 用于辐射诱发致癌细胞的定量研究 利用杂交的人类细胞进行转化。为此提出了一种方法 涉及使用单克隆性细胞检测恶性转化细胞 一种肿瘤特异性膜蛋白的抗体及荧光激活 细胞分类。