ULTRASOUND AND MALIGNANT TRANSFORMATION IN VITRO

超声与体外恶性转化

• 批准号: 3179892
• 负责人: GEORGE H HARRISON
• 金额: $ 9.33万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-07-01 至 1990-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3179892
• 关键词: X ray; benzopyrenes; bioassay; cancer risk; ionizing radiation; laboratory mouse; particle beam; phorbols; physical chemical interaction; radiation carcinogenesis; radiation sensitivity; tissue /cell culture; tumor promoters; ultrasound biological effect

We propose to assess the oncogenic risk of ultrasound, using the C3H/10T1/2 in vitro assay for malignant transformation. This assay is a more direct and quantitative measure of oncogenic risk than are assays of mutation or chromosomal alterations. Pulsed ultrasonic beams configured to simulate diagnostic conditions will be used to irradiate cells alone, in combination with carcinogenic treatment, or followed by post-treatment culturing in 0.1 /g/ml of 12-0-tetradecanoyl-phorbol-13-acetate (TPA) in order to investigate the possible oncogenic interaction of co-stressors and tumor promotors with ultrasound. In examining the effect of ultrasound alone, experiments will be designed to detect a malignant transformation rate equivalent to or greater than the response of 1 Gy of 250 kVp X-rays or to about 2 /g/ml of benzo(a)pyrene, with 95% statistical confidence above the spontaneous transformation rate. In examining the effect of ultrasound as a co-stressor, experiments will be designed to detect a two-fold enhancement of transformation by ultrasound with 95% statistical confidence. Cells will be insonated in suspension in a transmission chamber at 37 degrees C using uniform, minimally focussed beam profiles, with insonation parameters simulating the upper range of pressure amplitudes from clinical ultrasonic imaging and doppler systems. Emphasis will be placed on providing replicable, well-characterized ultrasound delivery and bioassay.

我们建议评估超声波的致癌风险，使用 C3H/10T1/2体外恶性转化实验。这 化验是一种更直接、更定量的致癌风险测量方法 而不是突变或染色体改变的检测。脉冲 配置为模拟诊断条件的超声波波束将 单独用于照射细胞，与致癌物质结合使用 处理，或随后处理后在0.1/g/ml的 12-0-十四酰-佛波醇-13-乙酸酯(TPA) 研究协同应激源和致癌因素可能的相互作用 肿瘤促进剂与超声波。 在单独检查超声波的效果时，实验将被 被设计成检测相当于 或大于1Gy250kVPX射线的响应，或 苯并(A)芘含量约为2/g/ml，统计置信度为95% 高于自发转化率。在研究 超声波作为共同应激源的作用，将设计实验 通过超声波检测两倍增强的转化 有95%的统计置信度。细胞会受到电离辐射 在37摄氏度的传输室中使用 均匀的、最小聚焦的光束轮廓，带有辐射 模拟压力幅值上限范围的参数 来自临床超声成像和多谱勒系统。重点 将放在提供可复制的、功能齐全的 超声波传递和生物检测。