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ULTRASOUND AND MALIGNANT TRANSFORMATION IN VITRO

超声与体外恶性转化

基本信息

批准号：
3179893
负责人：
GEORGE H HARRISON
金额：
$ 10.21万
依托单位：
UNIVERSITY OF MARYLAND BALTIMORE
依托单位国家：
美国
项目类别：
财政年份：
1985
资助国家：
美国
起止时间：
1985-07-01 至 1991-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3179893
关键词：
X ray benzopyrenes bioassay cancer risk ionizing radiation laboratory mouse particle beam phorbols physical chemical interaction radiation carcinogenesis radiation sensitivity tissue /cell culture tumor promoters ultrasound biological effect

项目摘要

We propose to assess the oncogenic risk of ultrasound, using the C3H/10T1/2 in vitro assay for malignant transformation. This assay is a more direct and quantitative measure of oncogenic risk than are assays of mutation or chromosomal alterations. Pulsed ultrasonic beams configured to simulate diagnostic conditions will be used to irradiate cells alone, in combination with carcinogenic treatment, or followed by post-treatment culturing in 0.1 /g/ml of 12-0-tetradecanoyl-phorbol-13-acetate (TPA) in order to investigate the possible oncogenic interaction of co-stressors and tumor promotors with ultrasound. In examining the effect of ultrasound alone, experiments will be designed to detect a malignant transformation rate equivalent to or greater than the response of 1 Gy of 250 kVp X-rays or to about 2 /g/ml of benzo(a)pyrene, with 95% statistical confidence above the spontaneous transformation rate. In examining the effect of ultrasound as a co-stressor, experiments will be designed to detect a two-fold enhancement of transformation by ultrasound with 95% statistical confidence. Cells will be insonated in suspension in a transmission chamber at 37 degrees C using uniform, minimally focussed beam profiles, with insonation parameters simulating the upper range of pressure amplitudes from clinical ultrasonic imaging and doppler systems. Emphasis will be placed on providing replicable, well-characterized ultrasound delivery and bioassay.

我们建议使用以下方法评估超声的致癌风险： C3H/10T1/2 体外恶性转化测定。这测定是致癌风险的更直接和定量的测量比突变或染色体改变的检测更重要。脉冲式配置为模拟诊断条件的超声波束将单独用于照射细胞，与致癌物质结合使用处理，或处理后以0.1/g/ml的浓度进行培养 12-0-十四烷酰基-佛波醇-13-乙酸酯 (TPA) 研究共同应激源可能的致癌相互作用超声肿瘤促进剂。在单独检查超声波的效果时，实验将旨在检测相当于的恶性转化率或大于 1 Gy 的 250 kVp X 射线响应或约 2 /g/ml 苯并(a)芘，统计置信度为 95% 高于自发转化率。在审查超声波作为辅助应激源的作用，将设计实验检测超声转化的两倍增强具有 95% 的统计置信度。细胞将被超声处理在 37°C 的传动室中悬浮，使用均匀、最小聚焦的光束轮廓，具有声波模拟压力幅值上限的参数来自临床超声成像和多普勒系统。强调将致力于提供可复制的、特征良好的超声传输和生物测定。