Advanced glycosaminoglycan sequencing

先进的糖胺聚糖测序

• 批准号: BB/L023717/1
• 负责人: Mark Skidmore
• 金额: $ 42.11万
• 依托单位: Keele University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FL023717%2F1
• 关键词: Advanced; glycosaminoglycan; sequencing

The interaction between proteins and cell-surface ligands underpin many biological processes such as cell growth, homeostasis, apoptosis and the ability of pathogens to invade host cells. One important family of cell surface ligands are the carbohydrate family of glycosaminoglycans (GAGs) found on the surface of almost all mammalian cells. Analysis of the sequence of the linear block-like arrangement of these GAGs is a significant technical challenge due to only miniscule quantities of pure material being (readily) available, poor chemistries and insensitive detection equipment. This research aims to develop and test a new method for sequencing these GAG polysaccharides utilising recent fundamental improvements made by the applicant. These developments include the exploitation of the opposite end of the molecule than is conventionally used (non-reducing end), with a vastly improved labelling mechanism and an advanced detection system for the conventional end of the molecule (reducing end). This approach will provide a powerful and sensitive sequencing technique capable of employing both ends of the GAG saccharide for characterization. The information gained from GAG sequencing will enable researchers to study previously elusive structures and processes that are biologically and medically significant.

蛋白质和细胞表面配体之间的相互作用支持许多生物过程，如细胞生长、稳态、凋亡和病原体入侵宿主细胞的能力。细胞表面配体的一个重要家族是糖胺聚糖（GAG）的碳水化合物家族，其存在于几乎所有哺乳动物细胞的表面上。这些GAG的线性嵌段状排列的序列的分析是一个重大的技术挑战，这是由于只有极少量的纯材料是（容易）可用的，化学性质差和检测设备不灵敏。本研究旨在开发和测试一种利用申请人最近进行的基本改进对这些GAG多糖进行测序的新方法。这些发展包括利用分子的与常规使用相反的末端（非还原末端），大大改进了标记机制和用于分子的常规末端（还原末端）的先进检测系统。该方法将提供一种强大且灵敏的测序技术，能够采用GAG糖的两端进行表征。从GAG测序中获得的信息将使研究人员能够研究以前难以理解的具有生物学和医学意义的结构和过程。

项目成果

Heparin prevents Zika virus induced-cytopathic effects in human neural progenitor cells.

• DOI: 10.1016/j.antiviral.2016.12.023
• 发表时间: 2017-04
• 期刊: Antiviral research
• 影响因子: 7.6
• 作者: Ghezzi S;Cooper L;Rubio A;Pagani I;Capobianchi MR;Ippolito G;Pelletier J;Meneghetti MCZ;Lima MA;Skidmore MA;Broccoli V;Yates EA;Vicenzi E
• 通讯作者: Vicenzi E

An Inexpensive, Pulsed, and Multiple Wavelength Bench-Top Light Source for Biological Spectroscopy

用于生物光谱学的廉价、脉冲、多波长台式光源

• DOI: 10.3390/plasma1010008
• 发表时间: 2018
• 期刊: Plasma
• 作者: Holman J

Molecular Changes in Dengue Envelope Protein Domain III upon Interaction with Glycosaminoglycans.

• DOI: 10.3390/pathogens9110935
• 发表时间: 2020-11-11
• 期刊: Pathogens (Basel, Switzerland)
• 作者: Hyatt JG;Prévost S;Devos JM;Mycroft-West CJ;Skidmore MA;Winter A
• 通讯作者: Winter A

Analysis of Heparin Samples by Attenuated Total Reflectance Fourier-Transform Infrared Spectroscopy in the Solid State.

• DOI: 10.1021/acscentsci.2c01176
• 发表时间: 2023-03-22
• 期刊: ACS CENTRAL SCIENCE
• 影响因子: 18.2
• 作者: Devlin, Anthony J.;Mycroft-West, Courtney J.;Turnbull, Jeremy E.;de Lima, Marcelo Andrade;Guerrini, Marco;Yates, Edwin A.;Skidmore, Mark A.
• 通讯作者: Skidmore, Mark A.

Heat dissipation of hybrid iron oxide-gold nanoparticles in an agar phantom

• DOI: 10.4172/2157-7439.1000335
• 发表时间: 2015-11
• 期刊: Journal of Nanomedicine & Nanotechnology
• 作者: A. Curtis;Maryam Malekigorji;J. Holman;M. Skidmore;Clare Hoskins