HUMAN PAPILLOMAVIRUSES IN GENITAL TRACT DYSPLASIAS

生殖道发育不良中的人乳头瘤病毒

• 批准号: 3182986
• 负责人: FELIX O WETTSTEIN
• 金额: $ 12.44万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-04-01 至 1989-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3182986
• 关键词: DNA methylation; Papillomavirus; aneuploidy; antiviral antibody; cervix neoplasms; genetic manipulation; genetic mapping; histology; human papillomavirus; human tissue; lysogeny; oncogenic virus; plasmids; preneoplastic state; serology /serodiagnosis; synthetic antigens; viral carcinogenesis; virus DNA; virus RNA; virus antigen; virus related neoplasm /cancer; warts

The long term goals of this research are to provide evidence for and an understanding of the role of human papilloma viruses in the development of cervical dysplasias and invasive carcinomas. The specific aims include: 1) Establish a correlation between HPV types DNA ploidy patterns and morphometric parameters seen in histologic sections. 2) a) Characterize the state of viral DNA. b) Map viral transcripts. c) Identify "early" viral proteins and elucidate their function in cervical carcinomas and carcinoma derived cell lines. 3) Investigate aspects of the immune response in papillomavirus infections. a) Determine the presence of antibodies to "early" viral proteins. b) Attempt to construct type specific antigens for "oncogenic" HPV"s and search for antibodies to these antigens in human sera which may provide an indication about the spread of these viruses in the human population.

这项研究的长期目标是为以下方面提供证据和 了解人乳头瘤病毒在发展中的作用 宫颈发育不良和浸润性癌。 具体目标包括： 1)建立HPV类型DNA倍体模式与 组织切片中观察到的形态学参数。 2)a）表征病毒DNA的状态。 B）绘制病毒转录物图谱。 c）鉴定“早期”病毒蛋白并阐明其在宫颈癌中的功能。 癌和癌衍生的细胞系。 3)研究乳头状瘤病毒感染的免疫反应。 a）确定“早期”病毒蛋白的抗体的存在。 B）尝试构建“致癌”HPV的型特异性抗原， 在人血清中寻找这些抗原的抗体， 这些病毒在人群中传播的迹象。