DEVELOPMENT OF VACCINES AGAINST PAPILLOMAVIRUSES

宫颈癌是全世界女性最常见的癌症之一， 据信，少量生殖器人类乳头瘤病毒在 在这些癌症的发展过程中起着至关重要的作用。发展中的 预防感染或有效治疗皮损的疫苗可能 大大降低了这些病毒造成的发病率和死亡率。因为 在乳头瘤病毒的狭窄宿主范围中，不可能产生 一个带有人类病毒的动物模型。我们的目标是用棉尾巴 兔乳头瘤病毒(CRPV)作为动物模型来测试策略 保护性和治疗性疫苗的开发。CRPV，作为人类的一部分 病毒，与癌症的发展有关。 具体目标是： 1)开发一种基于使用过的李斯特菌的独特的新疫苗系统 并在CRPV系统中进行测试。 2)构建表达病毒结构蛋白的重组痘苗病毒 构象表位的最佳维持基因及其表达 病毒蛋白和IL2用于增加T细胞刺激。 3)用重组杆状病毒或在 用于亚单位疫苗开发的酵母。 4)评价开发多肽疫苗的可能性。

Cervical cancer is one of the most common cancers in women worldwide and it is believed that a small number of genital human papillomaviruses play an essential role in the development of these cancers. Development of vaccines to protect from infection or effective in therapy of lesions could greatly reduce morbidity and mortality caused by these viruses. Because of the narrow host range of papillomaviruses, it is not possible to develop a animal model with the human viruses. Our goal is to use cottontail rabbit papillomavirus (CRPV) as an animal model to test strategies for protective and therapeutic vaccine development. CRPV, as some of the human viruses, is associated with the development of cancers. The specific aims are: 1) Develop a unique novel system for vaccines based on the used Listeria monocytogenes and test it in the CRPV system. 2) Construct recombinant vaccinia viruses expressing both viral structural genes for optimal maintenance of conformational epitopes and expressing viral protein and IL2 for increased T cell stimulation. 3) Express viral structural proteins with recombinant bacuolovirus or in yeast for subunit vaccine development. 4) Evaluate the possibility to develop a peptide vaccine.