CHOROIDAL VESSEL DESTRUCTION TO TREAT OCULAR MELANOMA

我们的目标是研究一种治疗脉络膜的新方法。 黑色素瘤。这项研究的目的是确定使用 选择性破坏脉络膜血的光动力疗法 脉络膜黑色素瘤的血管。有大量证据表明 光动力疗法(PDT)可以有效地破坏两者的血管 肿瘤组织和正常组织。因此，有可能 提供脉络膜黑色素瘤的脉络膜血管床可以选择性地 用经巩膜光导的光动力疗法破坏(在治疗中 周围性肿瘤)或经瞳孔投光治疗 光凝性光动力疗法(用于治疗后部肿瘤)。两者都是光传递 将对程序进行评估。 在所有光动力疗法研究中使用的卟啉是Photofrin II和红光 (630 Nm)将被用于 光敏纤维蛋白的光化学活化--Ⅱ.有色兔和 无色素性格林黑色素瘤(移植到脉络膜)将是我们的 动物和肿瘤模型。这项研究建议的具体目标是： 1)确定生产国产化所需的PDT处理参数 和完全脉络膜血管破坏，并记录类型和 眼部急性和慢性毒性程度(包括眼内和眼外 由这些处理引起的。眼底镜观察， 眼底照相、荧光素血管造影术和组织病理学 将进行检查；2)评估PDT的能力(指导 脉络膜血管)成功破坏脉络膜 黑色素瘤。肿瘤再生长参数、肿瘤治愈措施和 将进行组织学评估；以及3)确定程度 光动力疗法引起的脉络膜血管破坏和脉络膜增强 实验兔吸氧增加时的肿瘤反应 在光动力疗法中的浓度。

Our objective is to examine a new approach for the treatment of choroidal melanoma. The research is designed to determine the efficacy of using porphyrin photodynamic therapy to selectively destroy the choroidal blood vessels supplying choroidal melanomas. There is considerable evidence that photodynamic therapy (PDT) can effectively destroy blood vessels in both tumor tissue and normal tissue. It is therefore possible that the choroidal vascular bed supplying choroidal melanomas can be selectively destroyed by PDT using either trans-scleral light delivery (in treating peripheral tumors) or by trans-pupillary light delivery for photocoagulative PDT (in treating posterior tumors). Both light delivery procedures will be evaluated. The porphyrin to be used in all PDT studies is Photofrin II and red light (630 nm) generated by an argon pumped dye laser will be used for photochemical activation of Photofrin II. The pigmented rabbit and the amelanotic Greene's melanoma (transplanted to the choroid) will be our animal and tumor model. The specific aims of this research proposal are: 1) to determine the PDT treatment parameters required to produce localized and complete choroidal vessel destruction, and to document the type and extent of acute and chronic ocular toxicity (both in and out of the treatment field) induced by these treatments. Ophthalmoscopic observation, fundus photography, fluorescein angiograph and histopathological examinations will be performed; 2) to assess the ability of PDT (directed at the choroidal blood vessels) to successfully destroy choroidal melanomas. Tumor regrowth parameters, tumor cure measurements and histological evaluations will be performed; and 3) to determine the degree of enhancement in PDT induced choroidal vessel destruction and choroid tumor response when experimental rabbits breathe increased oxygen concentrations during PDT.