CHOROIDAL VESSEL DESTRUCTION TO TREAT OCULAR MELANOMA

我们的目的是研究一种新的方法来治疗脉络膜 黑素瘤 这项研究旨在确定使用 选择性破坏脉络膜血液的卟啉光动力疗法 供应脉络膜黑色素瘤的血管。 有相当多的证据表明 光动力疗法（PDT）可以有效地破坏血管， 肿瘤组织和正常组织。 因此， 脉络膜血管床供应脉络膜黑色素瘤可以选择性 使用经巩膜光输送（在治疗中）被PDT破坏 外周肿瘤）或通过经瞳孔光递送， 光凝PDT（治疗后部肿瘤）。 两种光传输 将对程序进行评估。 在所有PDT研究中使用的卟啉是Photofrin II和红光 (630将使用氩泵浦的染料激光器产生的波长为20 nm的激光脉冲， Photofrin II的光化学活化。 色素兔和 无色素性格林黑色素瘤（移植到脉络膜）将是我们的 动物和肿瘤模型。 这项研究建议的具体目标是： 1)以确定产生局部化的PDT治疗参数， 和完全脉络膜血管破坏，并记录类型和 急性和慢性眼毒性的程度（包括在 治疗场）。 检眼镜观察， 眼底照相、荧光素血管造影和组织病理学检查 将进行检查; 2）评估PDT的能力（直接 在脉络膜血管处）以成功地破坏脉络膜 黑素瘤。 肿瘤再生长参数、肿瘤治愈测量和 将进行组织学评价;以及3）确定 增强PDT诱导的脉络膜血管破坏和脉络膜 实验性兔吸氧后的肿瘤反应 PDT期间的浓度。

Our objective is to examine a new approach for the treatment of choroidal melanoma. The research is designed to determine the efficacy of using porphyrin photodynamic therapy to selectively destroy the choroidal blood vessels supplying choroidal melanomas. There is considerable evidence that photodynamic therapy (PDT) can effectively destroy blood vessels in both tumor tissue and normal tissue. It is therefore possible that the choroidal vascular bed supplying choroidal melanomas can be selectively destroyed by PDT using either trans-scleral light delivery (in treating peripheral tumors) or by trans-pupillary light delivery for photocoagulative PDT (in treating posterior tumors). Both light delivery procedures will be evaluated. The porphyrin to be used in all PDT studies is Photofrin II and red light (630 nm) generated by an argon pumped dye laser will be used for photochemical activation of Photofrin II. The pigmented rabbit and the amelanotic Greene's melanoma (transplanted to the choroid) will be our animal and tumor model. The specific aims of this research proposal are: 1) to determine the PDT treatment parameters required to produce localized and complete choroidal vessel destruction, and to document the type and extent of acute and chronic ocular toxicity (both in and out of the treatment field) induced by these treatments. Ophthalmoscopic observation, fundus photography, fluorescein angiograph and histopathological examinations will be performed; 2) to assess the ability of PDT (directed at the choroidal blood vessels) to successfully destroy choroidal melanomas. Tumor regrowth parameters, tumor cure measurements and histological evaluations will be performed; and 3) to determine the degree of enhancement in PDT induced choroidal vessel destruction and choroid tumor response when experimental rabbits breathe increased oxygen concentrations during PDT.