CHOROIDAL VESSEL DESTRUCTION TO TREAT OCULAR MELANOMA

脉络膜血管破坏治疗眼部黑色素瘤

• 批准号: 3187500
• 负责人: CHARLES Joseph GOMER
• 金额: $ 13.82万
• 依托单位: CHILDREN'S HOSPITAL OF LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-06-01 至 1991-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3187500
• 关键词: disease /disorder model; eye fundus photography; eye laser surgery; eye neoplasms; fluorescein angiography; histopathology; laboratory rabbit; melanoma; neoplasm /cancer therapy; nonvisual photosensitivity; ophthalmoscopy; oxygen consumption; photochemistry; photocoagulation therapy; porphyrins; uvea disorder

Our objective is to examine a new approach for the treatment of choroidal melanoma. The research is designed to determine the efficacy of using porphyrin photodynamic therapy to selectively destroy the choroidal blood vessels supplying choroidal melanomas. There is considerable evidence that photodynamic therapy (PDT) can effectively destroy blood vessels in both tumor tissue and normal tissue. It is therefore possible that the choroidal vascular bed supplying choroidal melanomas can be selectively destroyed by PDT using either trans-scleral light delivery (in treating peripheral tumors) or by trans-pupillary light delivery for photocoagulative PDT (in treating posterior tumors). Both light delivery procedures will be evaluated. The porphyrin to be used in all PDT studies is Photofrin II and red light (630 nm) generated by an argon pumped dye laser will be used for photochemical activation of Photofrin II. The pigmented rabbit and the amelanotic Greene's melanoma (transplanted to the choroid) will be our animal and tumor model. The specific aims of this research proposal are: 1) to determine the PDT treatment parameters required to produce localized and complete choroidal vessel destruction, and to document the type and extent of acute and chronic ocular toxicity (both in and out of the treatment field) induced by these treatments. Ophthalmoscopic observation, fundus photography, fluorescein angiograph and histopathological examinations will be performed; 2) to assess the ability of PDT (directed at the choroidal blood vessels) to successfully destroy choroidal melanomas. Tumor regrowth parameters, tumor cure measurements and histological evaluations will be performed; and 3) to determine the degree of enhancement in PDT induced choroidal vessel destruction and choroid tumor response when experimental rabbits breathe increased oxygen concentrations during PDT.

我们的目的是研究一种治疗脉络膜的新方法

项目成果

Aromatase gene expression and its exon I usage in human breast tumors. Detection of aromatase messenger RNA by reverse transcription-polymerase chain reaction.

芳香酶基因表达及其外显子 I 在人类乳腺肿瘤中的使用。

• DOI: 10.1016/s0960-0760(96)00100-8
• 发表时间: 1996
• 期刊: The Journal of steroid biochemistry and molecular biology
• 作者: Zhou,C;Zhou,D;Esteban,J;Murai,J;Siiteri,PK;Wilczynski,S;Chen,S
• 通讯作者: Chen,S

Tissue distribution and photosensitizing properties of mono-L-aspartyl chlorin e6 in a mouse tumor model.

• 发表时间: 1990-07
• 期刊: Cancer research
• 影响因子: 11.2
• 作者: C. Gomer;A. Ferrario

Systemic toxicity in mice induced by localized porphyrin photodynamic therapy.

• 发表时间: 1990-02
• 期刊: Cancer research
• 影响因子: 11.2
• 作者: A. Ferrario;C. Gomer

Differential cell photosensitivity following porphyrin photodynamic therapy.

卟啉光动力疗法后细胞光敏性的差异。

• 发表时间: 1988
• 期刊: Cancer research
• 影响因子: 11.2
• 作者: Gomer,CJ;Rucker,N;Murphree,AL
• 通讯作者: Murphree,AL