课题基金 / 基金详情

ABNORMAL TERMINAL TRANSFERASE BIOSYNTHESIS IN REMISSION

ABNORMAL TERMINAL TRANSFERASE BIOSYNTHESIS IN REMISSION
缓解期末端转移酶生物合成异常
批准号:
3184275
负责人:
ALLEN Edgar SILVERSTONE
金额:
$12.01万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1985
资助国家:
美国
项目状态:
已结题
起止时间:
1985-09-30 至 1988-08-31

项目摘要

项目成果

ALLEN Edgar SILVERSTONE的其他基金

相关文献

中文摘要
翻译
本研究的目的是开发和利用检测方法
英文摘要
The objective of this research is the development and utilization of assays to detect the presence of 0.5 to 1% leukemic cells in the total nucleated bone marrow cell population by utilizing the differential rate of synthesis of the template independent DNA polymerase terminal deoxynucleotidyl transferase (TdT). Rather than utilizing the already well-characterized steady-state measurements embodies in biochemical, immunofluoresecent, and antibody affinity assays, the dynamic quality of rate of TdT biosynthesis will be utilized to discriminate between normal and leukemic TdT-positive marrow poulations. The biosynthesis rate by leukemic cells is measurably greater than the rate of synthesis by equivalent numbers of normal TdT positive cells in bone marrow. As a further refinement, cDNA probes for human TdT-specific message will be utilized to establish correlations beweeen rate of RNA synthesis, steady-state levels of mRNA, and protein biosynthesis. These measurements will be extended to heterogeneous model populations and clinical samples. The prognostic value of these assays will be determned by applying the assays to routine clincal samples from leukemic patients in treatment, or off treatment and in remission, who have had, or might develop, TdT-positive disease.
期刊论文(4)
专著(0)
科研奖励(0)
会议论文
Measurement of terminal deoxynucleotidyl transferase mRNA in clinical samples: a new parameter in analysis of leukemia cells.
临床样本中末端脱氧核苷酸转移酶 mRNA 的测量:白血病细胞分析的新参数。
DOI: 10.1002/ajh.2830250305
发表时间: 1987
期刊: American journal of hematology
影响因子: 12.8
作者: [Wolf,SC, Steinherz,PG, Landau,NR, Silverstone,AE]
通讯作者: Silverstone,AE
DOI: 10.4049/jimmunol.144.4.1169
发表时间: 1990-02
期刊: Journal of immunology
影响因子: 4.4
作者: [J. S. Fine;A. Silverstone;T. Gasiewicz]
通讯作者: J. S. Fine;A. Silverstone;T. Gasiewicz
Prothymocyte activity is reduced by perinatal 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure.
围产期接触 2,3,7,8-四氯二苯并-对二恶英会降低胸腺细胞活性。
DOI: --
发表时间: 1990
期刊: The Journal of pharmacology and experimental therapeutics
影响因子: --
作者: [Fine,JS, Gasiewicz,TA, Fiore,NC, Silverstone,AE]
通讯作者: Silverstone,AE
DOI: --
发表时间: 1989
期刊: Molecular pharmacology
影响因子: 3.6
作者: [J. S. Fine;T. Gasiewicz;A. Silverstone]
通讯作者: J. S. Fine;T. Gasiewicz;A. Silverstone
Flow Cytometer
  • 批准号:
    6442234
  • 项目类别:
  • 资助金额:
    $32.6万
  • 财政年份:
    2002
  • 负责人:
    ALLEN Edgar SILVERSTONE
  • 依托单位:
CONSEQUENCES OF THYMIC ATROPHY INDUCED BY TCDD AND E2
  • 批准号:
    2704531
  • 项目类别:
  • 资助金额:
    $28.6万
  • 财政年份:
    1994
  • 负责人:
    ALLEN Edgar SILVERSTONE
  • 依托单位:
CONSEQUENCES OF THYMIC ATROPHY INDUCED BY TCDD AND E2
  • 批准号:
    2156422
  • 项目类别:
  • 资助金额:
    $21.51万
  • 财政年份:
    1994
  • 负责人:
    ALLEN Edgar SILVERSTONE
  • 依托单位:
CONSEQUENCES OF THYMIC ATROPHY INDUCED BY TCDD AND E2
  • 批准号:
    2156423
  • 项目类别:
  • 资助金额:
    $20.73万
  • 财政年份:
    1994
  • 负责人:
    ALLEN Edgar SILVERSTONE
  • 依托单位: