Flow Cytometer

描述（由申请人提供）：我们重新提交的申请 申请资金购买一台带有双通道的FACS Vantage/SE流式细胞仪， 激光器，以取代9岁的大量使用的流式细胞仪星星加仪器与 单激光工作台（无法升级）和19年前的相干模型 90/5氩激光。我们需要一个新设计的气溶胶控制装置， 额外的PMT管、克隆附件和Turbosort射流， 这一要求。这种新的流式细胞仪将是必不可少的工作15 目前，NIH资助的受资助者中有20人获得了NIH的资助， 有5份申请提交审查的额外研究者。这 流式细胞仪/细胞分选仪将是纽约中部唯一的此类仪器。 有一个10岁的库尔特史诗双激光分选机在康奈尔大学非常 有限的一般用途，以及与我们所要求的仪器相当的仪器 在奥尔巴尼和罗切斯特有限量供应。因为它的年龄和 维修问题以及其技术限制，我们将在我们的贸易 FACStar Plus和Laser，以降低这种新仪器的成本。调查人员 来自CNY地区其他机构的人员将能够使用该工具， 包括来自伊萨卡康奈尔大学的研究人员。技术支持和 空间将通过国家和机构资金管理提供 通过病理学系。至少有12名调查员需要 激光器，9个将需要额外的PMT管，6个将需要脉冲处理器，5个将 需要克隆附件，12个需要排序功能，4个需要 生物危害气溶胶控制。 我们已经解决了以前的评论者的有益批评， 我们的研究计划与我们所要求的仪器和激光器相匹配。我们 机构和病理学系增加了他们的承诺， 这一设施和文书（在裁决的情况下），以便它将充分 支持和维护。

DESCRIPTION (provided by applicant): This resubmission of our application requests funds for the purchase of a FACS Vantage/SE flow Cytometer with dual lasers to replace a 9 year old heavily used FACS Star Plus instrument with a single laser bench (that cannot be upgraded) and a 19 year old Coherent Model 90/5 argon laser. We are requesting a newly designed aerosol containment unit, additional PMT tubes, a cloning attachment, and the Turbosort fluidics as part of this request. This new flow cytometer would be essential to the work of 15 currently NIH funded grantees with 20 NIH grants between them, and for 3 additional investigators who have 5 applications submitted for review. This Flow Cytometer/Cell Sorter will be the only such instrument in Central NY. There is a 10 year old Coulter Epics dual laser sorter at Cornell with very limited general use, and instruments equivalent to the one we are requesting available on a limited basis in Albany and Rochester. Because of its age and repair problems as well as its technical limitations, we will trade in our FACStar Plus and Laser to reduce the cost of this new instrument. Investigators from other institutions in the CNY region will have access to this instrument, including researchers from Cornell University in Ithaca. Technical support and space will be provided through State and Institutional funding administered through the Department of Pathology. At least 12 investigators need the dual laser, 9 will need additional PMT tubes, 6 will need Pulse Processors, 5 will need the cloning attachment, 12 will need sorting capabilities, and 4 will need Biohazard Aerosol containment. We have addressed the helpful criticisms of the previous reviewers, matching our research plans to the instrument and lasers we are requesting. Our institution and the Department of Pathology have increased their commitments to this facility and instrument (in event of award), so that it will be adequately supported and maintained.