Flow Cytometer

流式细胞仪

• 批准号: 6442234
• 负责人: ALLEN Edgar SILVERSTONE
• 金额: $ 32.6万
• 依托单位: UPSTATE MEDICAL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2004-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6442234
• 关键词: biomedical equipment purchase; flow cytometry

DESCRIPTION (provided by applicant): This resubmission of our application requests funds for the purchase of a FACS Vantage/SE flow Cytometer with dual lasers to replace a 9 year old heavily used FACS Star Plus instrument with a single laser bench (that cannot be upgraded) and a 19 year old Coherent Model 90/5 argon laser. We are requesting a newly designed aerosol containment unit, additional PMT tubes, a cloning attachment, and the Turbosort fluidics as part of this request. This new flow cytometer would be essential to the work of 15 currently NIH funded grantees with 20 NIH grants between them, and for 3 additional investigators who have 5 applications submitted for review. This Flow Cytometer/Cell Sorter will be the only such instrument in Central NY. There is a 10 year old Coulter Epics dual laser sorter at Cornell with very limited general use, and instruments equivalent to the one we are requesting available on a limited basis in Albany and Rochester. Because of its age and repair problems as well as its technical limitations, we will trade in our FACStar Plus and Laser to reduce the cost of this new instrument. Investigators from other institutions in the CNY region will have access to this instrument, including researchers from Cornell University in Ithaca. Technical support and space will be provided through State and Institutional funding administered through the Department of Pathology. At least 12 investigators need the dual laser, 9 will need additional PMT tubes, 6 will need Pulse Processors, 5 will need the cloning attachment, 12 will need sorting capabilities, and 4 will need Biohazard Aerosol containment. We have addressed the helpful criticisms of the previous reviewers, matching our research plans to the instrument and lasers we are requesting. Our institution and the Department of Pathology have increased their commitments to this facility and instrument (in event of award), so that it will be adequately supported and maintained.

描述（由申请人提供）：我们重新提交的申请 请求资金购买具有双通道功能的 FACS Vantage/SE 流式细胞仪 激光将使用了 9 年的大量使用的 FACS Star Plus 仪器替换为 单激光工作台（无法升级）和 19 年历史的相干模型 90/5 氩激光。我们需要一个新设计的气溶胶遏制装置， 额外的 PMT 管、克隆附件和 Turbosort 射流作为一部分 这个请求。这种新型流式细胞仪对于 15 名科学家的工作至关重要 目前 NIH 为受资助者提供了 20 项 NIH 资助，其中 3 项 其他调查员已提交 5 份申请供审查。这 流式细胞仪/细胞分选仪将是纽约中部唯一的此类仪器。 康奈尔大学有一台已有 10 年历史的 Coulter Epics 双激光分选机，性能非常好 有限的一般用途，以及与我们要求的相当的仪器 奥尔巴尼和罗切斯特有限提供。由于其年龄和 维修问题及其技术限制，我们将在我们的 FACStar Plus 和 Laser 降低了这种新仪器的成本。调查员 人民币地区其他机构的人员将可以使用该工具， 包括伊萨卡康奈尔大学的研究人员。技术支持和 空间将通过国家和机构管理的资金提供 通过病理科。至少 12 名调查员需要双重身份 激光，9 个需要额外的 PMT 管，6 个需要脉冲处理器，5 个需要 需要克隆附件，12 个需要分拣功能，4 个需要 生物危害气溶胶遏制。 我们已经解决了之前审稿人的有益批评，匹配 我们对我们所要求的仪器和激光器的研究计划。我们的 机构和病理学系加大了对 该设施和仪器（如果获奖），以便充分利用 支持和维护。