CYTOGENETICS AND MOLECULAR GENETICS OF GYNECOLOGIC TUMOR

该项目的一个长期目标是提供信息， 研究妇科肿瘤的染色体异常， 这些肿瘤中的“特异性”和“常见”异常。 具体 染色体异常现在具有诊断意义， 常见的可能提供显著的独立预后 信息. 此外，位点特异性染色体变化将识别 这些染色体片段包含的基因可能对 恶性发展。 目的是确定 染色体研究在妇科肿瘤中的应用 可以积累足够的数据。 手术切除的肿瘤 来自生殖器官的物质，以及渗出液， 可用于这项研究。 细胞遗传学结果将增加和 与病人现有的电脑资料比较， 临床状况和病程。 两者的研究 肿瘤组织和积液提供了比较 原发和转移部位的核型改变。 癌症的 子宫颈癌发生之前会有一系列癌前病变。 宫颈各种病变的细胞遗传学检查结果可能是最重要的 对了解染色体变化很有价值， 肿瘤发生 每个标本都进行常规染色体分析， 直接培养和短期培养。 成分确定的培养基，含 使用成纤维细胞抑制剂，例如精胺， 的细胞系。 外源性和自体饲养层 将在需要时用于启动肿瘤细胞生长。 染色体制备按标准进行 协议. 小区同步过程有时需要 以提高有丝分裂的产量。 当一个特定的染色体 发现重排与特定肿瘤相关， 我们将采用原位染色体杂交。 南部 分析，和脉冲场梯度电泳，以研究 候选基因参与这种疾病。 细胞癌基因扩增可作为预后指标， 癌的 我们计划确定其扩增的基因， 与某些妇科肿瘤密切相关。 广泛原位 特殊固定和处理的肿瘤的杂交分析 进行具有许多生长相关基因探针的组织， 并将有助于选择最合适的基因， 分析. DNA分析的结论性结果之后， 通过以下方式表征可能的异常转录本： 北方和核酸酶S1保护分析。

One long-term objective of this project is to provide information on chromosome abnormalities in gynecologic tumors, and to find "specific" and "common" abnormalities in these tumors. Specific chromosome abnormalities now have diagnostic implications and the common ones may provide significant independent prognostic information. Also, site-specific chromosomal changes will identify the chromosomal segments containing genes which may be crucial to the malignant development. The intention is to determine the usefulness of chromosome studies in gynecologic tumors when sufficient data can be accumulated. Surgically removed tumor materials from reproductive organs, as well as effusions, are available for this study. Cytogenetic findings will add to and compare with the existing computerized information on the patients' clinical status and the course of their diseases. Study of both tumor tissue and effusion offers the opportunity of comparing the karyotypic changes in primary and metastatic sites. Caner of the uterine cervix is preceded by a spectrum of precancerous changes. Cytogenetic findings of various lesions of the cervix could be most valuable in the understanding of chromosomal changes in tumorigenesis. Each specimen is routinely processed for chromosome analysis, both directly and after short-term culture. Defined medium containing a fibroblast inhibitor, e.g. spermine, is used for the development of cell lines in culture. Exogeneous and autologous feeder layers will be used to initiate tumor cell growth when they are needed. Chromosome preparation is carried out according to standard protocols. Cell synchronization procedures are sometimes performed to enhance the yield of mitosis. When a specific chromosome rearrangement is found to be associated with a particular tumor, we will employ in situ chromosomal hybridization. Southern analysis, and pulsed field gradient electrophoresis to study the involvement of candidate gene(s) in this disorder. Amplification of cellular oncogenes could be a prognostic index in cancers. We plan to identify the gene(s) whose amplification is closely related to certain gynecologic tumors. Extensive in situ hybridization analyses of specially fixed and processed tumor tissues with numbers of growth related gene probes are performed and will help to select the most appropriate gene(s) for DNA analysis. Conclusive results of DNA analysis will be followed by the characterization of possible abnormal transcripts by means of Northern and nuclease S1 protection analysis.