CYTOGENETICS AND MOLECULAR GENETICS OF GYNECOLOGIC TUMOR

该项目的一个长期目标是提供信息 关于妇科肿瘤的染色体异常，并发现 这些肿瘤中有“特殊的”和“常见的”异常。特定的 染色体异常现在具有诊断意义， 常见的肿瘤可能会提供显著的独立预后 信息。此外，特定部位的染色体变化将识别 染色体片段包含的基因可能对 恶性发展。其目的是确定 染色体研究在妇科肿瘤中的应用 可以积累足够的数据。手术切除的肿瘤 来自生殖器官的物质以及渗出物是 可用于本研究。细胞遗传学的发现将增加和 与现有计算机化的患者信息进行比较 临床状况和病程。对两者的研究 肿瘤组织和积液提供了比较 原发灶和转移灶的核型改变。《癌症》 子宫颈先有一系列癌前病变。 宫颈各种病变的细胞遗传学表现可能是最多的 对了解人类染色体的变化有重要意义 肿瘤发生学。 每个样本都经过常规处理以进行染色体分析， 直接培养和短期培养后。定义的介质包含 一种成纤维细胞抑制物，如精胺，用于发育 培养中的细胞系。外源性和自体饲养层 将被用来在需要时启动肿瘤细胞的生长。 染色体制备按标准进行 协议。有时执行信元同步过程 以提高有丝分裂的产率。当一条特定的染色体 重排被发现与特定的肿瘤有关， 我们将采用染色体原位杂交技术。南方 分析，并用脉冲梯度电泳法研究 候选基因(S)参与本病。 细胞癌基因扩增可作为判断预后的指标 癌症。我们计划确定其扩增产物为 与某些妇科肿瘤密切相关。广泛的原地 特殊固定和加工的肿瘤的杂交分析 具有多个生长相关基因探针的组织被进行 这将有助于选择最适合DNA的基因(S 分析。DNA分析的最终结果将随后公布 对可能的异常转录本的表征 Northern和核酸酶S1保护分析。

One long-term objective of this project is to provide information on chromosome abnormalities in gynecologic tumors, and to find "specific" and "common" abnormalities in these tumors. Specific chromosome abnormalities now have diagnostic implications and the common ones may provide significant independent prognostic information. Also, site-specific chromosomal changes will identify the chromosomal segments containing genes which may be crucial to the malignant development. The intention is to determine the usefulness of chromosome studies in gynecologic tumors when sufficient data can be accumulated. Surgically removed tumor materials from reproductive organs, as well as effusions, are available for this study. Cytogenetic findings will add to and compare with the existing computerized information on the patients' clinical status and the course of their diseases. Study of both tumor tissue and effusion offers the opportunity of comparing the karyotypic changes in primary and metastatic sites. Caner of the uterine cervix is preceded by a spectrum of precancerous changes. Cytogenetic findings of various lesions of the cervix could be most valuable in the understanding of chromosomal changes in tumorigenesis. Each specimen is routinely processed for chromosome analysis, both directly and after short-term culture. Defined medium containing a fibroblast inhibitor, e.g. spermine, is used for the development of cell lines in culture. Exogeneous and autologous feeder layers will be used to initiate tumor cell growth when they are needed. Chromosome preparation is carried out according to standard protocols. Cell synchronization procedures are sometimes performed to enhance the yield of mitosis. When a specific chromosome rearrangement is found to be associated with a particular tumor, we will employ in situ chromosomal hybridization. Southern analysis, and pulsed field gradient electrophoresis to study the involvement of candidate gene(s) in this disorder. Amplification of cellular oncogenes could be a prognostic index in cancers. We plan to identify the gene(s) whose amplification is closely related to certain gynecologic tumors. Extensive in situ hybridization analyses of specially fixed and processed tumor tissues with numbers of growth related gene probes are performed and will help to select the most appropriate gene(s) for DNA analysis. Conclusive results of DNA analysis will be followed by the characterization of possible abnormal transcripts by means of Northern and nuclease S1 protection analysis.