THE ROLE OF OMEGA-3 PUFA IN CANCER PREVENTION

OMEGA-3 多不饱和脂肪酸在预防癌症中的作用

• 批准号: 3190370
• 负责人: SUSAN M FISCHER
• 金额: $ 13.03万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-03-01 至 1991-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3190370
• 关键词: DNA replication; arachidonate; benzanthracenes; benzoates; cancer prevention; carcinogenesis inhibitor; cell differentiation; cell growth regulation; chemical binding; chemical carcinogen; chemical carcinogenesis; delayed hypersensitivity; fatty acid metabolism; inflammation; laboratory mouse; mitogens; mixed lymphocyte reaction test; natural killer cells; nitrosoguanidines; nutrient drug interaction; nutrient intake activity; nutrition aspect of cancer; nutrition related neoplasm /cancer; nutrition related tag; omega 3 fatty acid; ornithine decarboxylase; phorbols; protein glutamine gamma glutamyltransferase; skin neoplasms; toxin metabolism; tumor promoters

The goals of this investigation are to determine whether isocaloric diets high in n-3 relative to n-6-fatty acids will have the chemopreventive effect in mouse skin carcainogenesis of reducing tumor initiation and promotion. It will be determined whether isocaloric diets containing 10% fat as either high n-6, n- 3, both or predominately saturated fatty acids alter tumor response to a metabolism-requiring carcinogen, 7,12- dimethylbenz(a)anthrene (DMBA) and a direct acting carcinogen, N-methyl-N-nitro-N-nitrosoquanidine (MNNG). Dietary effects on carcinogen binding to DNA and metabolite profiles in the epidermis will also be measured. The effect of these diets will also be studied with respect to tumor promotion, using 12-0- tetradecanyolphorbol-13-acetate (TPA) and benzoyl peroxide, as well as several parameters believed to be important to the promotion process: hyperplasia, inflammation and ornithine decarboxylase induction. Since it is expected that high n-6 fatty acid diets will enhance promotion, tumor experiments will be done in which the major arachidonate metabolites are applied to mice on the high n-3 fatty acid diets to attempt to partially restore promotion. Studies will also be carried out to determine whether the fatty acid effects seen above are epidermal in origin rather than due to changes in systemic parameters such as the immune system. This will be done by culturing mouse epidermal cells in a defined medium containing n-6 and/or n-3 fatty acids and measuring several parameters of growth and differentiation. Finally, it will also be determined if the different fatty acid diets affect the responsiveness of the immune system to challenge. Several parameters will be measured: delayed hypersensitivity, mitogen responsiveness, mixed lymphocyte reactions and natural killer cell activity.

这项调查的目的是确定是否