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The Bacterial Invasion Port of Bdellovibrio

蛭弧菌的细菌入侵口

基本信息

批准号：
BB/M010325/1
负责人：
Renee Sockett
金额：
$ 52.75万
依托单位：
University of Nottingham
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2015
资助国家：
英国
起止时间：
2015 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FM010325%2F1
关键词：
Bacterial Invasion Port Bdellovibrio

项目摘要

There is a great need to find new ways to destroy bacterial pathogens now that many are becoming resistant to antibiotics and antibacterials. It is important to prevent plant crops and farm animals against diseases, as well as to protect human health. Some predatory bacteria that are harmless to humans naturally kill bacterial pathogens by building a molecular "port" in them and entering through this port to kill them. Importantly, because this access port is build from several molecular parts, it is not easy for the pathogens to adapt to not fit one of the parts and so escape being invaded. Also the things that are pumped through the port are good at attacking bacterial membranes- there arent many antibiotics that do that. Because Bdellovibrio only attack bacteria they don't harm humans or their membranes.We recently identified some of the parts of the natural invasion port of the predatory bacterium Bdellovibrio, and some control proteins that work with the port. We now want to find out how those parts work together to build and co-ordinate what the port does so we know how bacterial membranes are invaded. Then we will be able to use all these parts to build a toolkit to make pathogenic bacteria be invaded and killed.In this project we are trying to find out what protein chemicals are pumped through the port into the bacteria and when, and what attaches the port to the bacteria while the predatory bacteria are pumping in the membrane damaging proteins.We can use a simple coloured test to see if a protein is damaging the membrane of a bacterium. If the membrane is damaged then the coloured dye goes pink. In this way we can test proteins coded by the DNA of the bacteria and see which make the dye go pink when exposed to bacteria, to see if they are damaged by each protein.We also think that some of the same proteins that pull the Bdellovibrio inside the bacteria can then do a transformation by bolting on different proteins and start to act as a pump to pump more degrading proteins into the bacteria to break them down. We will test this too.From the project we will learn how to tackle pathogenic bacteria in farms or in public or in hospital buildings by attacking their outer membranes with Bdellovibrio-derived materials. These could go on to help as working antibiotics become scarcer.

由于许多细菌病原体对抗生素和抗菌药物产生耐药性，因此迫切需要找到新的方法来消灭细菌病原体。预防植物作物和农场动物疾病以及保护人类健康非常重要。一些对人类无害的捕食性细菌通过在细菌病原体中建立分子“端口”并通过该端口进入以杀死细菌病原体来自然杀死细菌病原体。重要的是，因为这个入口是由几个分子部分组成的，所以病原体不容易适应其中一个部分，从而逃避入侵。此外，通过端口泵送的东西善于攻击细菌膜-没有多少抗生素可以做到这一点。由于蛭弧菌只攻击细菌，它们不会伤害人类或他们的膜。我们最近确定了捕食性细菌蛭弧菌的自然入侵端口的一些部分，以及与端口一起工作的一些控制蛋白质。我们现在想知道这些部分是如何协同工作来构建和协调端口的功能的，这样我们就知道细菌膜是如何被入侵的。然后我们将能够使用所有这些部件来构建一个工具包，使致病细菌被入侵和杀死。在这个项目中，我们试图找出什么蛋白质化学物质通过端口泵入细菌，以及何时，以及当捕食性细菌将破坏细胞膜的蛋白质泵入时，是什么将端口连接到细菌上。我们可以使用一个简单的颜色测试来查看蛋白质是否正在破坏细胞膜指细菌。如果膜被损坏，那么有色染料就会变成粉红色。通过这种方法，我们可以检测由细菌DNA编码的蛋白质，看看是什么使染料在暴露于细菌时变成粉红色，看看它们是否被每种蛋白质破坏。我们还认为，一些将蛭弧菌拉入细菌内部的相同蛋白质可以通过与不同的蛋白质结合来进行转化，并开始充当泵，将更多降解蛋白质泵入细菌中，以分解它们。我们也将对此进行测试。从该项目中，我们将学习如何通过使用蛭弧菌衍生材料攻击它们的外膜来对付农场、公共场所或医院建筑中的致病菌。随着有效抗生素越来越少，这些药物可能会继续发挥作用。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Interrupting peptidoglycan deacetylation during Bdellovibrio predator-prey interaction prevents ultimate destruction of prey wall, liberating bacterial-ghosts.

DOI：
10.1038/srep26010
发表时间：
2016-05-23
期刊：
Scientific reports
影响因子：
4.6
作者：
Lambert C;Lerner TR;Bui NK;Somers H;Aizawa S;Liddell S;Clark A;Vollmer W;Lovering AL;Sockett RE
通讯作者：
Sockett RE

Dynamics of chromosome replication and its relationship to predatory attack lifestyles in Bdellovibrio bacteriovorus

食菌蛭弧菌染色体复制动态及其与捕食性攻击生活方式的关系

DOI：
10.1101/519983
发表时间：
2019
期刊：
影响因子：
0
作者：
Makowski L
通讯作者：
Makowski L

Evolutionary diversification of the RomR protein of the invasive deltaproteobacterium, Bdellovibrio bacteriovorus.

入侵性δ变形菌、噬菌蛭弧菌的 RomR 蛋白的进化多样化。

DOI：
10.1038/s41598-019-41263-5
发表时间：
2019
期刊：
Scientific reports
影响因子：
4.6
作者：
Lowry RC
通讯作者：
Lowry RC

Fluorescent D-amino-acids reveal bi-cellular cell wall modifications important for Bdellovibrio bacteriovorus predation.

DOI：
10.1038/s41564-017-0029-y
发表时间：
2017-12
期刊：
Nature microbiology
影响因子：
28.3
作者：
Kuru E;Lambert C;Rittichier J;Till R;Ducret A;Derouaux A;Gray J;Biboy J;Vollmer W;VanNieuwenhze M;Brun YV;Sockett RE
通讯作者：
Sockett RE

Ankyrin-mediated self-protection during cell invasion by the bacterial predator Bdellovibrio bacteriovorus.

DOI：
10.1038/ncomms9884
发表时间：
2015-12-02
期刊：
Nature communications
影响因子：
16.6
作者：
Lambert C;Cadby IT;Till R;Bui NK;Lerner TR;Hughes WS;Lee DJ;Alderwick LJ;Vollmer W;Sockett RE;Lovering AL
通讯作者：
Lovering AL