ANTITUMOR AGENTS FROM HIGHER PLANTS

描述（研究者摘要）：本研究项目旨在 抗肿瘤新药的分离和结构鉴定 已证实具有生物活性的高等植物。 预筛选 生物测定基于针对人类肿瘤的疾病导向细胞毒性 细胞系：A-549（非小细胞肺癌）、HT-29（结肠癌）和 MCF-7（乳腺癌）。活性组分的分级和纯化 将通过选定的生物测定指导组分。 的结构 新化合物将通过现代光谱方法阐明， 包括高场二维NMR、固态NMR、质谱 光谱学和X射线晶体学。 所有活性化合物将被 提交给国家癌症研究所进行进一步的体外人 肿瘤细胞组以及体内异种移植物评估。 期间 在调查过程中，继续收集和预筛选 将从台湾、北美和印度引进更多的工厂 以确保新的活跃植物的可靠供应。

DESCRIPTION (Investigator's Abstract): This research project is aimed at the isolation and structural elucidation of novel antitumor agents from higher plants which have confirmed bioactivities. The prescreening bioassays are based on disease-oriented cytotoxicity against human tumor cell lines: A-549 (non-small cell lung cancer), HT-29 (colon cancer), and MCF-7 (breast cancer). Fractionation and purification of active constituents will be directed by selected bioassay. The structures of novel compounds will be elucidated by modern spectroscopic methods, including high field two-dimensional NMR, solid state NMR, mass spectrometry and X-ray crystallography. All active compounds will be submitted to the National Cancer Institute for further in vitro human tumor cell panels as well as in vivo xenograft evaluations. During the course of this investigation, continuing collection and prescreening of additional plants from Taiwan, North America, and India will be carried out to ensure reliable supplies of new active plants.